Mycobacterial phage D29 holin of 141 aas, Gp11. Gp11 shows a lipid concentration-dependent conformational switch from an α-helix to a β-sheet structure (Lella and Mahalakshmi 2013). Toxicity has been studied showing that TMS 1 (but not TMS 2) and a C-terminal coiled-coil region are essential for activity because the latter is necessary for holin aggregation, insertion into the membrane and bacterial cell death (Kamilla and Jain 2015). A role for TMS 2 in pore formation has been proposed (Lella and Mahalakshmi 2016). The first TMS has been engineered to form a nanopore (Lella and Mahalakshmi 2016). The D29 and Chy1 phage holins are identical in amino acid sequence (Gan et al. 2016).
|Protein Name:||Gene 11 protein|
|Species:||Mycobacterium phage D29  |
|Number of TMSs:||2|
1: MSPKIRETLY YVGTLVPGIL GIALIWGGID AGAAANIGDI VAGALNLVGA AAPATAAVKV
61: NQQRKDGTLT TSPVDQVTRG VEQVLAAKQN AEAEVERVKQ ALESAVNGAV PQLGPLASQI
121: LNGIQPAYSQ PFDPHTQPWN R