TCID | Name | Domain | Kingdom/Phylum | Protein(s) |
---|---|---|---|---|

*1.E.36.1.1 | Putative holin, Gp33 of 127 aas and 2 TMSs | Viruses |
Caudovirales | Gp33 of Mycobacterial phage Cjw1 |

*1.E.36.1.2 | Putative holin Gp36 of 84 aas and 2 TMSs (Catalão | Viruses |
Caudovirales | Gp36 of mycobacterial phage PBI1 |

*1.E.36.1.3 | Viruses |
Caudovirales | Gp10 of mycobacterial phage Timshel | |

*1.E.36.1.4 | Putative holin, Gp29 of 134 aas and 2 TMSs | Viruses |
Caudovirales | Gp29 of mycobacterial phage Charlie |

*1.E.36.1.5 | Putative holin Gp14 of 144 aas and 2 TMSs | Viruses |
Caudovirales | Gp14 of mycobacterial phage EricB |

*1.E.36.1.6 | Putative holin of 146 aas and 2 TMSs | Bacteria |
Actinobacteria | Putative holin of Mycobacterium massiliense |

*1.E.36.1.7 | Mycobacterial phage D29 holin of 141 aas, Gp11. Gp11 shows a lipid concentration-dependent conformational switch from an α-helix to a β-sheet structure (Lella and Mahalakshmi 2013). Toxicity has been studied showing that TMS 1 (but not TMS 2) and a C-terminal coiled-coil region are essential for activity because the latter is necessary for holin aggregation, insertion into the membrane and bacterial cell death (Kamilla and Jain 2015). A role for TMS 2 in pore formation has been proposed (Lella and Mahalakshmi 2016). The first TMS has been engineered to form a nanopore (Lella and Mahalakshmi 2016). The D29 and Chy1 phage holins are identical in amino acid sequence (Gan | Viruses |
Caudovirales | Holin, gp11 of Mycobacterial phage D29. |

*1.E.36.1.8 | Putative holin of 138 aas and 2 TMSs | Bacteria |
Actinobacteria | Putative holin of Mycobacterium abscessus |

*1.E.36.1.9 | Holin of 136 aas and 2 TMSs, gp255. | Viruses |
Caudovirales | gp255 of Mycobacterium phage Pleione |

*1.E.36.1.10 | Uncharacterized protein of 145 aas and 4 TMSs. | Bacteria |
Actinobacteria | UP of Demequina flava |

*1.E.36.2.1 | Putative holin of 69 aas and 2 TMSs | Bacteria |
Actinobacteria | Putative holin of Actinomyces neuii |

*1.E.36.2.2 | Viruses |
Caudovirales | Gp31 of mycobacterial phage Brujita | |

*1.E.36.2.3 | Putative holin of 67 aas and 2 TMSs | Viruses |
Caudovirales | Gp27 of mycobacterial phage Che9c |

*1.E.36.2.4 | Putative holin of 68 aas and 2 TMSs | Bacteria |
Actinobacteria | Putative holin of Mobiluncus mulieris |

*1.E.36.3.1 | Holin of 64 aas and 2 TMSs, HolB (Delisle | Viruses |
Caudovirales | HolB of phage Ar-1 |

*1.E.36.4.1 | Putative holin of 178 aas and 2 TMSs, Gp48 | Viruses |
Caudovirales | Gp48 and mycobacterial phage Ares |

*1.E.36.5.1 | Viruses |
Caudovirales | Gp54 of Mycobacterial phage Omega | |

*1.E.36.5.2 | Putative holin of 98 aas and 2 TMSs, Gp71 | Viruses |
Caudovirales | Gp71 of mycobacterial phage Corndog |

*1.E.36.6.1 | Putative holin, Gp17 of 144 aas and 4 TMSs | Viruses |
Caudovirales | Gp17 of Mycobacterial phage Daisy |

*1.E.36.6.2 | Putative holin of 113 aas and 4 TMSs | Bacteria |
Actinobacteria | Putative holin of Nocardia farcinica |

*1.E.36.6.3 | Putative holin, Gp16 of 135 aas and 4 TMSs | Viruses |
Caudovirales | Gp16 of mycobacterial phage Acadian |

*1.E.36.6.4 | Putative holin, Gp32 of 150 aas and 4 TMSs | Viruses |
Caudovirales | Gp32 of Mycobacterial phage Larva |

*1.E.36.6.5 | Putative holin, Gp31 of 128 aas and ~3 TMSs | Viruses |
Caudovirales | Gp31 of Mycobacterial phage TM4 |