TCDB is operated by the Saier Lab Bioinformatics Group
TCIDNameDomainKingdom/PhylumProtein(s)
*1.E.36.1.1









Putative holin, Gp33 of 127 aas and 2 TMSs

Viruses
Caudovirales
Gp33 of Mycobacterial phage Cjw1
*1.E.36.1.2









Putative holin Gp36 of 84 aas and 2 TMSs (Catalão et al. 2012).

Viruses
Caudovirales
Gp36 of mycobacterial phage PBI1
*1.E.36.1.3









Putative holin Gp10 of 137 aas and 2 or 3 TMSs

Viruses
Caudovirales
Gp10 of mycobacterial phage Timshel
*1.E.36.1.4









Putative holin, Gp29 of 134 aas and 2 TMSs

Viruses
Caudovirales
Gp29 of mycobacterial phage Charlie
*1.E.36.1.5









Putative holin Gp14 of 144 aas and 2 TMSs

Viruses
Caudovirales
Gp14 of mycobacterial phage EricB
*1.E.36.1.6









Putative holin of 146 aas and 2 TMSs

Bacteria
Actinobacteria
Putative holin of Mycobacterium massiliense
*1.E.36.1.7









Mycobacterial phage D29 holin of 141 aas, Gp11.  Gp11 shows a lipid concentration-dependent conformational switch from an α-helix to a β-sheet structure (Lella and Mahalakshmi 2013). Toxicity has been studied showing that TMS 1 (but not TMS 2) and a C-terminal coiled-coil region are essential for activity because the latter is necessary for holin aggregation, insertion into the membrane and bacterial cell death (Kamilla and Jain 2015).  A role for TMS 2 in pore formation has been proposed (Lella and Mahalakshmi 2016).  The first TMS has been engineered to form a nanopore (Lella and Mahalakshmi 2016).  The D29 and Chy1 phage holins are identical in amino acid sequence (Gan et al. 2016).

Viruses
Caudovirales
Holin, gp11 of Mycobacterial phage D29.
*1.E.36.1.8









Putative holin of 138 aas and 2 TMSs

Bacteria
Actinobacteria
Putative holin of Mycobacterium abscessus
*1.E.36.1.9









Holin of 136 aas and 2 TMSs, gp255.

Viruses
Caudovirales
gp255 of Mycobacterium phage Pleione
*1.E.36.1.10









Uncharacterized protein of 145 aas and 4 TMSs.

Bacteria
Actinobacteria
UP of Demequina flava
*1.E.36.2.1









Putative holin of 69 aas and 2 TMSs

Bacteria
Actinobacteria
Putative holin of Actinomyces neuii
*1.E.36.2.2









Putative holin of 67 aas and 2 TMSs, Gp31 of mycobacterial phage Brujita

Viruses
Caudovirales
Gp31 of mycobacterial phage Brujita
*1.E.36.2.3









Putative holin of 67 aas and 2 TMSs

Viruses
Caudovirales
Gp27 of mycobacterial phage Che9c
*1.E.36.2.4









Putative holin of 68 aas and 2 TMSs

Bacteria
Actinobacteria
Putative holin of Mobiluncus mulieris
*1.E.36.3.1









Holin of 64 aas and 2 TMSs, HolB (Delisle et al. 2006).

Viruses
Caudovirales
HolB of phage Ar-1
*1.E.36.4.1









Putative holin of 178 aas and 2 TMSs, Gp48

Viruses
Caudovirales
Gp48 and mycobacterial phage Ares
*1.E.36.5.1









Putative holin of 106 aas and 2 TMSs, Gp54 (Catal√£o et al. 2012).

Viruses
Caudovirales
Gp54 of Mycobacterial phage Omega
*1.E.36.5.2









Putative holin of 98 aas and 2 TMSs, Gp71

Viruses
Caudovirales
Gp71 of mycobacterial phage Corndog
*1.E.36.6.1









Putative holin, Gp17 of 144 aas and 4 TMSs

Viruses
Caudovirales
Gp17 of Mycobacterial phage Daisy
*1.E.36.6.2









Putative holin of 113 aas and 4 TMSs

Bacteria
Actinobacteria
Putative holin of Nocardia farcinica
*1.E.36.6.3









Putative holin, Gp16 of 135 aas and 4 TMSs

Viruses
Caudovirales
Gp16 of mycobacterial phage Acadian
*1.E.36.6.4









Putative holin, Gp32 of 150 aas and 4 TMSs

Viruses
Caudovirales
Gp32 of Mycobacterial phage Larva
*1.E.36.6.5









Putative holin, Gp31 of 128 aas and ~3 TMSs

Viruses
Caudovirales
Gp31 of Mycobacterial phage TM4