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1.E.4.1.4
Holin of 134 aas and 4 TMSs.  The lysis genes encode the holin, peptidase M15A or endolysin, lysB and lysC. Each gene and combinations were cloned into Escherichia coli and the lytic effects were measured. Co-expression of holin and peptidase M15A showed the highest lysis activity. Expression of holin, lysB/C or holin, peptidase M15A, lysB and lysC lysed the E. coli membrane whereas peptidase M15A alone did not. The predicted transmembrane structures of holin and lysB/C indicated they could insert into the bacterial membrane to form pores, affecting cell permeability and causing lysis (Khakhum et al. 2016).

Accession Number:R4JJW4
Protein Name:Uncharacterized protein
Length:134
Molecular Weight:14364.00
Species:Burkholderia phage ST79 [1282994]
Number of TMSs:4
Substrate endolysin

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FASTA formatted sequence
1:	MQNLIRYRWV WLAVVWPPSA WAATVTFGDD LSSIPLAAVT LCLFLSFIGG LASTLQKLAA 
61:	DVAPVRSIGL EIAKDLVVSL VAGLLAFFAS EWMNFQAVLE AGVITLAGYG GSRVLDRALD 
121:	RALREVDRGA DAGR