Holin of 134 aas and 4 TMSs. The lysis genes encode the holin,
peptidase M15A or endolysin, lysB and lysC. Each gene and combinations
were cloned into Escherichia coli and the lytic effects were measured.
Co-expression of holin and peptidase M15A showed the highest lysis activity. Expression of holin, lysB/C or holin,
peptidase M15A, lysB and lysC lysed the E. coli membrane whereas
peptidase M15A alone did not. The predicted transmembrane structures of holin and lysB/C indicated they could insert into the bacterial membrane to
form pores, affecting cell permeability and causing lysis (Khakhum et al. 2016).
|Protein Name:||Uncharacterized protein|
|Species:||Burkholderia phage ST79  |
|Number of TMSs:||4|
1: MQNLIRYRWV WLAVVWPPSA WAATVTFGDD LSSIPLAAVT LCLFLSFIGG LASTLQKLAA
61: DVAPVRSIGL EIAKDLVVSL VAGLLAFFAS EWMNFQAVLE AGVITLAGYG GSRVLDRALD
121: RALREVDRGA DAGR