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1.E.8.1.1
Lysis protein, T, of 218 aas and 1 TMS. T holin activity is regulated by the largely periplasmic anti-holin RI (P13304) and the cytoplasmic antiholin RIII which act synergistically (Chen and Young 2016). RI is degraded by DegP (Tran et al., 2007).  Mutations in all 3 topological domains if RI (the N-terminal cytoplasmic domain, the single TMS, and the C-terminal periplasmic domain) can abrogate holin function (Moussa et al. 2014).

Accession Number:P06808
Protein Name:VLYS aka T aka RV
Length:218
Molecular Weight:25176.00
Species:Bacteriophage T4 [10665]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Host membrane1 / Single-pass membrane protein2
Substrate endolysin

Cross database links:

RefSeq: NP_049865.1   
Entrez Gene ID: 1258610   
Pfam: PF11031   

Gene Ontology

GO:0033644 C:host cell membrane
GO:0016021 C:integral to membrane
GO:0016998 P:cell wall macromolecule catabolic process
GO:0019835 P:cytolysis

References (4)

[1] “Nucleotide sequence of gene t (lysis gene) of the E. coli phage T4.”  Montag D.et.al.   3628006
[2] “Bacteriophage T4 genome.”  Miller E.S.et.al.   12626685
[3] “Receptor-recognizing proteins of T-even type bacteriophages. Constant and hypervariable regions and an unusual case of evolution.”  Montag D.et.al.   2958637
[4] “The asiA gene of bacteriophage T4 codes for the anti-sigma 70 protein.”  Orsini G.et.al.   8416914

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAAPRISFSP SDILFGVLDR LFKDNATGKV LASRVAVVIL LFIMAIVWYR GDSFFEYYKQ 
61:	SKYETYSEII EKERTARFES VALEQLQIVH ISSEADFSAV YSFRPKNLNY FVDIIAYEGK 
121:	LPSTISEKSL GGYPVDKTMD EYTVHLNGRH YYSNSKFAFL PTKKPTPEIN YMYSCPYFNL 
181:	DNIYAGTITM YWYRNDHISN DRLESICAQA ARILGRAK