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1.N.3.1.3
Male gamete fusion factor, HAP-2 of 742 aas and at least 2 TMSs.  HAP2 is expressed in all seven mating types of T. thermophila and fertility is only blocked when the gene is deleted from both cells of a mating pair. HAP2 deletion strains of complementary mating types can recognize one another and form pairs, but pair stability is compromised and membrane pore formation at the nuclear exchange junction is blocked (Cole et al. 2014). Cole et al. 2014 proposed a model in which each of the several hundred membrane pores established at the conjugation junction of mating Tetrahymena represents the equivalent of a male/female interface, and that pore formation is driven on both sides of the junction by the presence of HAP2.

Accession Number:Q22C76
Protein Name:Male gamete fusion factor
Length:742
Molecular Weight:82663.00
Species:Tetrahymena thermophila (strain SB210) [312017]
Number of TMSs:2
Substrate small molecules, macromolecules

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKFLAFGLIY FHFCILNRCE YITSSTIQKC YNSSNEPNNC SQKAVIVLSL ENGQIANTEQ 
61:	VVATLNQLSD SGVNKQLQNS FIFEVTKSPV TALFPLIYLQ DFNSQPLEQV IATTLFSCKD 
121:	GFYDSSPTCK FQYDSKGQKI LDSQGYCCYC SLSDILGMGN DLSRGKVCYA LNLGAGSATA 
181:	HCLKFSPLWY SAFKIQQYQL YFEVNINIYT VDSQNQKNLK QTLKLSTSNP TMKSSDNSTI 
241:	SKIIGTFTPT QPPADLSSYY LVKPSFPATD PRVLQGISSW MFVDKTMFTL DGTQCNKIGV 
301:	SYSGFRQQSS SCSQPVGSCL QNQLENLYQS DLILLSQNKQ PKYLLESQGN FNQVQFQGQT 
361:	ILQQGLSGSA STLITIEIDA AQIKFVTNLG IGCISQCSIN NFESHSGNGK LVALVQNQGN 
421:	YSAEFVLGFN CSSNVQPIQG QKLFLTANQL YNFNCSVSVN SDISAINNNC TINLYDAIGN 
481:	QLDSKNILFN TTSTNHTSNQ GNNTGQQQSS QEYKSSQSCS DKCSSFWSFW CYFSAGCIKE 
541:	AFKSIASIAG VASALALVIF LAKNGYLVPI IRFLCCCCCK SKKKENEKNK DKTDKKSIQE 
601:	SCSYDRSCCS HSISQSYQVE NKNKYKRSKI QRSFSSESCQ DKSKKIINEL SNLEETFEAN 
661:	KLYANIDKNS SIFEYFGFKK SFTFILYERN DILFLPQNST ILDMIGALQP QKGSYLAQKF 
721:	LEIVNKNALK VVSTSPLYLL IE