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2.A.1.1.13
Fructose uniporter, GLUT5.  The proteins from rat and cow have been crystalized and their structures have been determined in the open outward- and open inward-facing conformations, respectively. On the basis of comparisons of the inward-facing structures of GLUT5 and human GLUT1, a ubiquitous glucose transporter, a single point mutation proved to be enough to switch the substrate-binding preference from fructose to glucose. A comparison of the substrate-free structures of GLUT5 with occluded substrate-bound structures of E. coli XylE suggested that, in addition to a global rocker-switch-like re-orientation of the bundles, local asymmetric rearrangements of carboxy-terminal transmembrane bundle helices, TM7 and TM10, underlie a 'gated-pore' transport mechanism (Nomura et al. 2015).  GLUT5 is preferentially used for fructose uptake under (near) anoxic glycolysis to avoid feedback inhibition of phosphofructokinase (Park et al. 2017).

Accession Number:P22732
Protein Name:Solute carrier family 2, facilitated glucose transporter member 5
Length:501
Molecular Weight:54974.00
Species: [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate fructose

Cross database links:

Genevestigator: P22732 P22732
eggNOG: prNOG16095 COG0477
HEGENOM: HBG744444 HOG000202871
RefSeq: NP_003030.1   
Entrez Gene ID: 6518   
Pfam: PF00083   
OMIM: 138230  gene
KEGG: hsa:6518   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005353 F:fructose transmembrane transporter activity
GO:0005355 F:glucose transmembrane transporter activity
GO:0005975 P:carbohydrate metabolic process
GO:0015755 P:fructose transport
GO:0015758 P:glucose transport
GO:0055085 P:transmembrane transport
GO:0044281 P:small molecule metabolic process

References (12)

[1] “Human facilitative glucose transporters. Isolation, functional characterization, and gene localization of cDNAs encoding an isoform (GLUT5) expressed in small intestine, kidney, muscle, and adipose tissue and an unusual glucose transporter pseudogene-like sequence (GLUT6).”  Kayano T.et.al.   1695905
[2] “Regulation of expression of the human fructose transporter (GLUT5) by cyclic AMP.”  Mahraoui L.et.al.   8037665
[3] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[4] “The DNA sequence and biological annotation of human chromosome 1.”  Gregory S.G.et.al.   16710414
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “Cluster analysis of an extensive human breast cancer cell line protein expression map database.”  Harris R.A.et.al.   11840567
[7] “Human facilitative glucose transporters. Isolation, functional characterization, and gene localization of cDNAs encoding an isoform (GLUT5) expressed in small intestine, kidney, muscle, and adipose tissue and an unusual glucose transporter pseudogene-like sequence (GLUT6).”  Kayano T.et.al.   1695905
[8] “Regulation of expression of the human fructose transporter (GLUT5) by cyclic AMP.”  Mahraoui L.et.al.   8037665
[9] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[10] “The DNA sequence and biological annotation of human chromosome 1.”  Gregory S.G.et.al.   16710414
[11] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[12] “Cluster analysis of an extensive human breast cancer cell line protein expression map database.”  Harris R.A.et.al.   11840567
Structure:
1YG1     

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FASTA formatted sequence
1:	MEQQDQSMKE GRLTLVLALA TLIAAFGSSF QYGYNVAAVN SPALLMQQFY NETYYGRTGE 
61:	FMEDFPLTLL WSVTVSMFPF GGFIGSLLVG PLVNKFGRKG ALLFNNIFSI VPAILMGCSR 
121:	VATSFELIII SRLLVGICAG VSSNVVPMYL GELAPKNLRG ALGVVPQLFI TVGILVAQIF 
181:	GLRNLLANVD GWPILLGLTG VPAALQLLLL PFFPESPRYL LIQKKDEAAA KKALQTLRGW 
241:	DSVDREVAEI RQEDEAEKAA GFISVLKLFR MRSLRWQLLS IIVLMGGQQL SGVNAIYYYA 
301:	DQIYLSAGVP EEHVQYVTAG TGAVNVVMTF CAVFVVELLG RRLLLLLGFS ICLIACCVLT 
361:	AALALQDTVS WMPYISIVCV ISYVIGHALG PSPIPALLIT EIFLQSSRPS AFMVGGSVHW 
421:	LSNFTVGLIF PFIQEGLGPY SFIVFAVICL LTTIYIFLIV PETKAKTFIE INQIFTKMNK 
481:	VSEVYPEKEE LKELPPVTSE Q