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2.A.1.1.19
Glucose transporter and low affinity sensor, Rgt2p (regulates glucose transport in conjunction with Snf3p). Rgt2 generates an intracellular signal in response to glucose that leads to inhibition of the Rgt1 transcriptional repressor and consequently to derepression of HXT genes encoding glucose transporters. They have unusually long C-terminal tails that bind to Mth1 and Std1, paralogous proteins that regulate the function of the Rgt1 transcription factor. Scharff-Poulsen et al. 2018 showed that the C-terminal tail of Rgt2 is not responsible for its inability to transport glucose. RGT2 mutations that cause constitutive signal generation alter evolutionarily-conserved amino acids in the transmembrane spanning regions involved in maintaining an outward-facing conformation or the substrate binding site. These mutations may cause Rgt2 to adopt inward-facing or occluded conformations that generate the glucose signal.

Accession Number:Q12300
Protein Name:RGT2 aka Rgt2p aka YDL138W aka D2160
Length:763
Molecular Weight:83159.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate glucose

Cross database links:

Genevestigator: Q12300
eggNOG: fuNOG04879
HEGENOM: HBG744444
DIP: DIP-2865N
RefSeq: NP_010143.1   
Entrez Gene ID: 851417   
Pfam: PF00083   
KEGG: sce:YDL138W   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005536 F:glucose binding
GO:0004872 F:receptor activity
GO:0022891 F:substrate-specific transmembrane transporte...
GO:0008643 P:carbohydrate transport
GO:0051594 P:detection of glucose
GO:0007165 P:signal transduction
GO:0055085 P:transmembrane transport

References (4)

[1] “Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV.”  Woelfl S.et.al.   8972577
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[3] “Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression.”  Oezcan S.et.al.   8901598
[4] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNDSQNCLRQ REENSHLNPG NDFGHHQGAE CTINHNNMPH RNAYTESTND TEAKSIVMCD 
61:	DPNAYQISYT NNEPAGDGAI ETTSILLSQP LPLRSNVMSV LVGIFVAVGG FLFGYDTGLI 
121:	NSITDMPYVK TYIAPNHSYF TTSQIAILVS FLSLGTFFGA LIAPYISDSY GRKPTIMFST 
181:	AVIFSIGNSL QVASGGLVLL IVGRVISGIG IGIISAVVPL YQAEAAQKNL RGAIISSYQW 
241:	AITIGLLVSS AVSQGTHSKN GPSSYRIPIG LQYVWSSILA VGMIFLPESP RYYVLKDELN 
301:	KAAKSLSFLR GLPIEDPRLL EELVEIKATY DYEASFGPST LLDCFKTSEN RPKQILRIFT 
361:	GIAIQAFQQA SGINFIFYYG VNFFNNTGVD NSYLVSFISY AVNVAFSIPG MYLVDRIGRR 
421:	PVLLAGGVIM AIANLVIAIV GVSEGKTVVA SKIMIAFICL FIAAFSATWG GVVWVVSAEL 
481:	YPLGVRSKCT AICAAANWLV NFTCALITPY IVDVGSHTSS MGPKIFFIWG GLNVVAVIVV 
541:	YFAVYETRGL TLEEIDELFR KAPNSVISSK WNKKIRKRCL AFPISQQIEM KTNIKNAGKL 
601:	DNNNSPIVQD DSHNIIDVDG FLENQIQSND HMIAADKGSG SLVNIIDTAP LTSTEFKPVE 
661:	HPPVNYVDLG NGLGLNTYNR GPPSIISDST DEFYEENDSS YYNNNTERNG ANSVNTYMAQ 
721:	LINSSSTTSN DTSFSPSHNS NARTSSNWTS DLASKHSQYT SPQ