TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.1.1.3
Xylose:H+ symporter.  Also transports and binds D-glucose and 6-bromo-6-deoxy-D-glucose.  The 3-d structure is known in three conformers, outward occluded, inward occluded and inward open (Sun et al. 2012: Quistgaard et al. 2013).  Most of the sugar-binding residues are conserved with the human Glut-1, 2, 3 and 4 homologues.  The coalescence of intramolecular tunnels and cavities has been postulated to account for facilitated diffusion of sugars Quistgaard et al. 2013).  Most of the sugar-binding residues are conserved with the human Glut-1, 2, 3 and 4 homologues.  The coalescence of intramolecular tunnels and cavities has been postulated to account for facilitated diffusion of sugars (Cunningham and Naftalin 2014).

Accession Number:P0AGF4
Protein Name:D-xylose-proton symporter XylE aka B4031
Length:491
Molecular Weight:53608.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Xylose, H+

Cross database links:

Genevestigator: P0AGF4
EchoBASE: EB1069
EcoGene: EG11076
eggNOG: COG0477
HEGENOM: HBG744444
RefSeq: AP_004532.1    NP_418455.1   
Entrez Gene ID: 948529   
Pfam: PF00083   
BioCyc: EcoCyc:XYLE-MONOMER    ECOL168927:B4031-MONOMER   
KEGG: ecj:JW3991    eco:b4031   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0022891 F:substrate-specific transmembrane transporte...
GO:0015293 F:symporter activity
GO:0008643 P:carbohydrate transport
GO:0055085 P:transmembrane transport

References (7)

[1] “The cloning and DNA sequence of the gene xylE for xylose-proton symport in Escherichia coli K12.”  Davis E.O.et.al.   2820984
[2] “Mammalian and bacterial sugar transport proteins are homologous.”  Maiden M.C.J.et.al.   3543693
[3] “Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.”  Blattner F.R.et.al.   8265357
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “3' end of the malEFG operon in E.coli: localization of the transcription termination site.”  Francoz E.et.al.   2836810
[7] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
4GBY   4GBZ   4GC0   4JA3   4JA4   4QIQ     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MNTQYNSSYI FSITLVATLG GLLFGYDTAV ISGTVESLNT VFVAPQNLSE SAANSLLGFC 
61:	VASALIGCII GGALGGYCSN RFGRRDSLKI AAVLFFISGV GSAWPELGFT SINPDNTVPV 
121:	YLAGYVPEFV IYRIIGGIGV GLASMLSPMY IAELAPAHIR GKLVSFNQFA IIFGQLLVYC 
181:	VNYFIARSGD ASWLNTDGWR YMFASECIPA LLFLMLLYTV PESPRWLMSR GKQEQAEGIL 
241:	RKIMGNTLAT QAVQEIKHSL DHGRKTGGRL LMFGVGVIVI GVMLSIFQQF VGINVVLYYA 
301:	PEVFKTLGAS TDIALLQTII VGVINLTFTV LAIMTVDKFG RKPLQIIGAL GMAIGMFSLG 
361:	TAFYTQAPGI VALLSMLFYV AAFAMSWGPV CWVLLSEIFP NAIRGKALAI AVAAQWLANY 
421:	FVSWTFPMMD KNSWLVAHFH NGFSYWIYGC MGVLAALFMW KFVPETKGKT LEELEALWEP 
481:	ETKKTQQTAT L