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2.A.1.10.1
Nucleoside porter, NupG.  Guanosine, inosine, cytidine and thymidine but not uridine, adenosine and xanthosine are transported (Patching et al. 2005).

Accession Number:P0AFF4
Protein Name:Nucleoside permease NupG aka B2964
Length:418
Molecular Weight:46389.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Inosine, Cytidine, Thymidine, Adenosine, Guanosine

Cross database links:

Genevestigator: P0AFF4
EchoBASE: EB0658
EcoGene: EG10664
eggNOG: COG0477
HEGENOM: HBG347023
RefSeq: AP_003521.1    NP_417439.4   
Entrez Gene ID: 946282   
BioCyc: EcoCyc:NUPG-MONOMER    ECOL168927:B2964-MONOMER   
KEGG: ecj:JW2932    eco:b2964   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005337 F:nucleoside transmembrane transporter activity
GO:0015858 P:nucleoside transport

References (4)

[1] “Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system.”  Westh Hansen S.E.et.al.   3311747
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNLKLQLKIL SFLQFCLWGS WLTTLGSYMF VTLKFDGASI GAVYSSLGIA AVFMPALLGI 
61:	VADKWLSAKW VYAICHTIGA ITLFMAAQVT TPEAMFLVIL INSFAYMPTL GLINTISYYR 
121:	LQNAGMDIVT DFPPIRIWGT IGFIMAMWVV SLSGFELSHM QLYIGAALSA ILVLFTLTLP 
181:	HIPVAKQQAN QSWTTLLGLD AFALFKNKRM AIFFIFSMLL GAELQITNMF GNTFLHSFDK 
241:	DPMFASSFIV QHASIIMSIS QISETLFILT IPFFLSRYGI KNVMMISIVA WILRFALFAY 
301:	GDPTPFGTVL LVLSMIVYGC AFDFFNISGS VFVEKEVSPA IRASAQGMFL MMTNGFGCIL 
361:	GGIVSGKVVE MYTQNGITDW QTVWLIFAGY SVVLAFAFMA MFKYKHVRVP TGTQTVSH