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2.A.1.12.1
The sialic acid porter, NanT.  N-acetylneuraminic acid (Neu5Ac) serves as a sole source of carbon and nitrogen for E. coli.  It is a mucus-derived carbon source in the mammalian gut. NanT can also take up and allow efficient growth on the related sialic acids, N-glycolylneuraminic acid (Neu5Gc) and 3-keto-3-deoxy-d-glycero-d-galactonononic acid (KDN) (Hopkins et al. 2013).

Accession Number:P41036
Protein Name:NanT aka B3224
Length:496
Molecular Weight:53551.00
Species:Escherichia coli [83333]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Sialic acid

Cross database links:

Genevestigator: P41036
EchoBASE: EB4145
EcoGene: EG20145
eggNOG: COG0477
HEGENOM: HBG417084
RefSeq: AP_003766.1    NP_417691.4   
Entrez Gene ID: 947740   
Pfam: PF07690    PF00083   
BioCyc: EcoCyc:NANT-MONOMER    ECOL168927:B3224-MONOMER   
KEGG: ecj:JW3193    eco:b3224   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015538 F:sialic acid:hydrogen ion symporter activity
GO:0015739 P:sialic acid transport
GO:0055085 P:transmembrane transport

References (4)

[1] “Derived structure of the putative sialic acid transporter from Escherichia coli predicts a novel sugar permease domain.”  Martinez J.et.al.   7592358
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSTTTQNIPW YRHLNRAQWR AFSAAWLGYL LDGFDFVLIA LVLTEVQGEF GLTTVQAASL 
61:	ISAAFISRWF GGLMLGAMGD RYGRRLAMVT SIVLFSAGTL ACGFAPGYIT MFIARLVIGM 
121:	GMAGEYGSSA TYVIESWPKH LRNKASGFLI SGFSVGAVVA AQVYSLVVPV WGWRALFFIG 
181:	ILPIIFALWL RKNIPEAEDW KEKHAGKAPV RTMVDILYRG EHRIANIVMT LAAATALWFC 
241:	FAGNLQNAAI VAVLGLLCAA IFISFMVQSA GKRWPTGVML MVVVLFAFLY SWPIQALLPT 
301:	YLKTDLAYNP HTVANVLFFS GFGAAVGCCV GGFLGDWLGT RKAYVCSLLA SQLLIIPVFA 
361:	IGGANVWVLG LLLFFQQMLG QGIAGILPKL IGGYFDTDQR AAGLGFTYNV GALGGALAPI 
421:	IGALIAQRLD LGTALASLSF SLTFVVILLI GLDMPSRVQR WLRPEALRTH DAIDGKPFSG 
481:	AVPFGSAKND LVKTKS