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2.A.1.13.4
The high affinity (17 μM) facilitated diffusion, riboflavin-regulated riboflavin uptake system, Mch5 (Reihl and Stolz, 2005)

Accession Number:Q08777
Protein Name:Mch5
Length:521
Molecular Weight:57566.00
Species:Saccharomyces cerevisiae (Baker's yeast)   [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate riboflavin

Cross database links:

Genevestigator: Q08777
eggNOG: fuNOG06281
HEGENOM: HBG396145
RefSeq: NP_014951.1   
Entrez Gene ID: 854483   
Pfam: PF07690   
KEGG: sce:YOR306C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0032217 F:riboflavin transporter activity
GO:0032218 P:riboflavin transport

References (5)

[1] “Sequence and analysis of a 36.2 kb fragment from the right arm of yeast chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1, SLY41, a putative transport ATPase, a putative ribosomal protein and an SNF2 homologue.”  Poirey R.et.al.   9153758
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.”  Dujon B.et.al.   9169874
[3] “The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane.”  Makuc J.et.al.   11536335
[4] “The monocarboxylate transporter homolog Mch5p catalyzes riboflavin (vitamin B2) uptake in Saccharomyces cerevisiae.”  Reihl P.et.al.   16204239
[5] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSDSLTPKD TIVPEEQTNQ LRQPDLDEDS IHYDPEADDL ESLETTASYA STSVSAKVYT 
61:	KKEVNKGTDI ESQPHWGENT SSTHDSDKEE DSNEEIESFP EGGFKAWVVT FGCFLGLIAC 
121:	FGLLNSTGVI ESHLQDNQLS SESVSTIGWL FSLFLFVCSA SCIISGTYFD RNGFRTIMIV 
181:	GTVFHVAGLF ATANSTKYWH FILSFAIVCG FGNGIVLSPL VSVPAHYFFK RRGTALAMAT 
241:	IGGSVGGVVF PIMLRSFFSM KSDTDPTYGF VWGIRTLGFL DLALLTLSII LVKERLPHVI 
301:	ENSKDGESRW RYILRVYILQ CFDAKAFLDM KYLFCVLGTV FSELSINSAL TYYGSYATSH 
361:	GISANDAYTL IMIINVCGIP GRWVPGYLSD KFGRFNVAIA TLLTLFIVMF VGWLPFGTNL 
421:	TNMYVISALY GFCSGSVFSL LPVCCGQISK TEEFGKRYST MYFVVGFGTL VGIPITGAII 
481:	SIKTTADYQH YIIFSGLATF VSAVCYIISR AYCVGFKWVR F