TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.1.13.6
Plasma membrane proton-linked monocarboxylate transporter, MCT4 (SLC16A3). Catalyzes the rapid plasma membrane transport of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.  Catalyzes lactate efflux from glycolyzing cells (Halestrap 2013).  Residues involved in high affinity inhibitors have been identified (Nancolas et al. 2015).  Forms a complex with binding partner, CD147/BSG, which regulates the transport activity (Fisel et al. 2015).

Accession Number:O15427
Protein Name:Monocarboxylate transporter 4
Length:465
Molecular Weight:49469.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Monocarboxylates, Lactate, pyruvate, Branched-chain oxo acids

Cross database links:

Genevestigator: O15427 O15427
eggNOG: prNOG19452 NOG321880
HEGENOM: HBG444740 HOG000280688
RefSeq: NP_001035887.1    NP_001035888.1    NP_004198.1   
Entrez Gene ID: 9123   
Pfam: PF07690   
Drugbank: Drugbank Link   
OMIM: 603877  gene
KEGG: hsa:9123    hsa:9123   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0015355 F:secondary active monocarboxylate transmembr...
GO:0015293 F:symporter activity
GO:0015718 P:monocarboxylic acid transport
GO:0015711 P:organic anion transport
GO:0055085 P:transmembrane transport
GO:0015629 C:actin cytoskeleton
GO:0031965 C:nuclear membrane
GO:0008028 F:monocarboxylic acid transmembrane transporter activity
GO:0015355 F:secondary active monocarboxylate transmembrane transporter activity
GO:0007596 P:blood coagulation
GO:0050900 P:leukocyte migration
GO:0006090 P:pyruvate metabolic process

References (12)

[1] “Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past.”  Price N.T.et.al.   9425115
[2] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen J.V.et.al.   17081983
[5] “Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.”  Daub H.et.al.   18691976
[6] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[7] “Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past.”  Price N.T.et.al.   9425115
[8] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[9] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[10] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen J.V.et.al.   17081983
[11] “Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.”  Daub H.et.al.   18691976
[12] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MGGAVVDEGP TGVKAPDGGW GWAVLFGCFV ITGFSYAFPK AVSVFFKELI QEFGIGYSDT 
61:	AWISSILLAM LYGTGPLCSV CVNRFGCRPV MLVGGLFASL GMVAASFCRS IIQVYLTTGV 
121:	ITGLGLALNF QPSLIMLNRY FSKRRPMANG LAAAGSPVFL CALSPLGQLL QDRYGWRGGF 
181:	LILGGLLLNC CVCAALMRPL VVTAQPGSGP PRPSRRLLDL SVFRDRGFVL YAVAASVMVL 
241:	GLFVPPVFVV SYAKDLGVPD TKAAFLLTIL GFIDIFARPA AGFVAGLGKV RPYSVYLFSF 
301:	SMFFNGLADL AGSTAGDYGG LVVFCIFFGI SYGMVGALQF EVLMAIVGTH KFSSAIGLVL 
361:	LMEAVAVLVG PPSGGKLLDA THVYMYVFIL AGAEVLTSSL ILLLGNFFCI RKKPKEPQPE 
421:	VAAAEEEKLH KPPADSGVDL REVEHFLKAE PEKNGEVVHT PETSV