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2.A.1.13.7
Monocarboxylate transporter-5 (MCT5 or SLC16A4; sometimes referred to as MCT4). Lactate transport via the MCT5 is non enzymatically stimulated by carbonic anhydrase II (Becker et al., 2010). MCTs require an ancillary 1TMS glycoprotein, either Embigin (Q6PCB8; TC# 8.A.23.1.2) or basigin (P35613; TC# 8.A.23.1.1) for plasma membrane localization (Ovens et al., 2010).  Upregulated in some cancers and maintains the metabolic phenotype of these cancer cells by mediating lactate efflux together with a proton, promoting pH homeostasis (Baltazar et al. 2014).  Also transports the chemotheraputic agent, 3-bromopyruvate (Baltazar et al. 2014).

Accession Number:O15374
Protein Name:Monocarboxylate transporter 5
Length:487
Molecular Weight:54022.00
Species: [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Monocarboxylates

Cross database links:

Genevestigator: O15374 O15374
eggNOG: prNOG09304 NOG285688
HEGENOM: HBG716123 HOG000059591
RefSeq: NP_004687.1   
Entrez Gene ID: 9122   
Pfam: PF07690   
Drugbank: Drugbank Link   
OMIM: 603878  gene
KEGG: hsa:9122    hsa:9122   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0008028 F:monocarboxylic acid transmembrane transport...
GO:0015293 F:symporter activity
GO:0015718 P:monocarboxylic acid transport
GO:0055085 P:transmembrane transport
GO:0008028 F:monocarboxylic acid transmembrane transporter activity

References (6)

[1] “Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past.”  Price N.T.et.al.   9425115
[2] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[3] “The DNA sequence and biological annotation of human chromosome 1.”  Gregory S.G.et.al.   16710414
[4] “Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past.”  Price N.T.et.al.   9425115
[5] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[6] “The DNA sequence and biological annotation of human chromosome 1.”  Gregory S.G.et.al.   16710414

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLKREGKVQP YTKTLDGGWG WMIVIHFFLV NVFVMGMTKT FAIFFVVFQE EFEGTSEQIG 
61:	WIGSIMSSLR FCAGPLVAII CDILGEKTTS ILGAFVVTGG YLISSWATSI PFLCVTMGLL 
121:	PGLGSAFLYQ VAAVVTTKYF KKRLALSTAI ARSGMGLTFL LAPFTKFLID LYDWTGALIL 
181:	FGAIALNLVP SSMLLRPIHI KSENNSGIKD KGSSLSAHGP EAHATETHCH ETEESTIKDS 
241:	TTQKAGLPSK NLTVSQNQSE EFYNGPNRNR LLLKSDEESD KVISWSCKQL FDISLFRNPF 
301:	FYIFTWSFLL SQLAYFIPTF HLVARAKTLG IDIMDASYLV SVAGILETVS QIISGWVADQ 
361:	NWIKKYHYHK SYLILCGITN LLAPLATTFP LLMTYTICFA IFAGGYLALI LPVLVDLCRN 
421:	STVNRFLGLA SFFAGMAVLS GPPIAGWLYD YTQTYNGSFY FSGICYLLSS VSFFFVPLAE 
481:	RWKNSLT