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2.A.1.14.12
Plasma membrane, high affinity biotin:H+ symporter, Vht1

Accession Number:P53241
Protein Name:Vht1 aka YGR065C
Length:593
Molecular Weight:69083.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Biotin

Cross database links:

Genevestigator: P53241
eggNOG: fuNOG08118
HEGENOM: HBG398100
DIP: DIP-5519N
RefSeq: NP_011579.1   
Entrez Gene ID: 852956   
Pfam: PF07690   
KEGG: sce:YGR065C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015225 F:biotin transporter activity
GO:0005515 F:protein binding
GO:0015293 F:symporter activity
GO:0015878 P:biotin transport

References (3)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.”  Tettelin H.et.al.   9169869
[2] “Identification of the plasma membrane H+-biotin symporter of Saccharomyces cerevisiae by rescue of a fatty acid-auxotrophic mutant.”  Stolz J.et.al.   10373489
[3] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTISNKSWRS YFPHLRKLPE DDQYLYSDDT NSSIIAEEEL HHSVDKSSKT DVTAETTAVE 
61:	PHPHNLRHDL PYEVRDEAGR KWWKYFDEFE YRVNKEYKKS RKWYEFLYPN HTTQTKAERR 
121:	LLYKLDIIIA LYFFMLCWSK SVDLNNYTNA YVSNMKEDLN MKGNDYVYTS TIANVGAIVF 
181:	QLPFMYLLPR FPSHIILPVM DLGWTWFTFA CYRANSLAEL RAYRFILSAF GAAYYPVSQY 
241:	ILGCWYAPDE INSRVCLFFC GQQLGSVTSG LLQSRIFKSL NGVHGLAGWR WMFLIDAIAI 
301:	SLPTAIIGFF VIPGVPSKCY SLFLTDEEIR IARARNKRNQ IKDGVDKSKL APLWSRKLWK 
361:	KVFCTPAFWV LVVFDTCSWN NMTAYSGSYT LWLKSNTKYS IAQVNNLSVI PACLGFAYVI 
421:	FCAFGADLFR CKWIFMVFAA IMNTVSCALL IKWDIPSKAK WYAFFTTYFS VAASPCLWSF 
481:	INDFLRFDPQ VKAITWIAIY SFSQSTYAWI PTLAWPTVES PRFKTGYTVS LIFGAIYGLW 
541:	TFVVLFFYKR NEKKHALGNG IILYDSNKGE ELPEFVKKNM EERDGYYYLK RSS