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2.A.1.14.13
Broad specificity brain synaptic vesicle anion:Na+ symporter (transports glutamate, phosphate, chloride, etc.)(BNPI, EAT-4, VGLUT1) Chloride and ketone bodies regulate VGLUT activities (Omote et al., 2011). 

Accession Number:Q62634
Protein Name:BNPI
Length:560
Molecular Weight:61665.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cytoplasmic vesicle1 / Multi-pass membrane protein2
Substrate glutamate, Cl-, phosphate

Cross database links:

Genevestigator: Q62634
eggNOG: roNOG12569
RefSeq: NP_446311.1   
Entrez Gene ID: 116638   
Pfam: PF07690   
KEGG: rno:116638   

Gene Ontology

GO:0030054 C:cell junction
GO:0016021 C:integral to membrane
GO:0030672 C:synaptic vesicle membrane
GO:0019717 C:synaptosome
GO:0005315 F:inorganic phosphate transmembrane transport...
GO:0015321 F:sodium-dependent phosphate transmembrane tr...
GO:0015293 F:symporter activity
GO:0006836 P:neurotransmitter transport
GO:0006817 P:phosphate transport
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (10)

[1] “Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter.”  Ni B.et.al.   8202535
[2] “Developmentally-regulated expression of tissue-specific splice variant of rat vesicular glutamate transporter 1 in retina and pineal gland.”  Nogami H.et.al.   16987242
[3] “Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons.”  Takamori S.et.al.   11001057
[4] “Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter.”  Bellocchio E.E.et.al.   10938000
[5] “The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons.”  Herzog E.et.al.   11698619
[6] “Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2).”  Takamori S.et.al.   11698620
[7] “The expression of vesicular glutamate transporters defines two classes of excitatory synapse.”  Fremeau R.T. Jr.et.al.   11502256
[8] “The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone.”  Gras C.et.al.   18278042
[9] “Molecular anatomy of a trafficking organelle.”  Takamori S.et.al.   17110340
[10] “Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging.”  Mackenzie B.et.al.   18080752

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP VVDCTCFGLP 
61:	RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG HVVVQKAQFN WDPETVGLIH 
121:	GSFFWGYIVT QIPGGFICQK FAANRVFGFA IVATSTLNML IPSAARVHYG CVIFVRILQG 
181:	LVEGVTYPAC HGIWSKWAPP LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV 
241:	YGSFGIFWYL FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT 
301:	SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV MTIIVPIGGQ 
361:	IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS KGVAISFLVL AVGFSGFAIS 
421:	GFNVNHLDIA PRYASILMGI SNGVGTLSGM VCPIIVGAMT KHKTREEWQY VFLIASLVHY 
481:	GGVIFYGVFA SGEKQPWAEP EEMSEEKCGF VGHDQLAGSD ESEMEDEVEP PGAPPAPPPS 
541:	YGATHSTVQP PRPPPPVRDY