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2.A.1.14.23
Vesicular glutamate transporter #3 (VGLUT3) [Its absence in mice causes sensorineural deafness and seizures]. 70% identical to VGLUT2 (TC# 2.A.1.14.16) (Gras et al., 2002). VGLUT1-3 concentrate glutamate into synaptic vesicles before its exocytotic release and contribute to the regulation of serotonergic transmission and anxiety (Amilhon et al., 2010). It may catalyze uptake of the neurotransmitter coupled with H+ export and K+ uptake (Farsi et al. 2016).

Accession Number:Q8BFU8
Protein Name:Vesicular glutamate transporter 3
Length:601
Molecular Weight:66148.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cytoplasmic vesicle1 / Multi-pass membrane protein2
Substrate glutamate, H+, K+

Cross database links:

Genevestigator: Q8BFU8
HEGENOM: HBG561587
RefSeq: NP_892004.1   
Entrez Gene ID: 216227   
Pfam: PF07690   
KEGG: mmu:216227   

Gene Ontology

GO:0030054 C:cell junction
GO:0016021 C:integral to membrane
GO:0030672 C:synaptic vesicle membrane
GO:0019717 C:synaptosome
GO:0005313 F:L-glutamate transmembrane transporter activity
GO:0015293 F:symporter activity
GO:0015813 P:L-glutamate transport
GO:0006836 P:neurotransmitter transport
GO:0007605 P:sensory perception of sound
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (6)

[1] “Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons.”  Schaefer M.K.-H.et.al.   12384506
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “Impairment of SLC17A8 encoding vesicular glutamate transporter-3, VGLUT3, underlies nonsyndromic deafness DFNA25 and inner hair cell dysfunction in null mice.”  Ruel J.et.al.   18674745
[4] “The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone.”  Gras C.et.al.   18278042
[5] “Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging.”  Mackenzie B.et.al.   18080752
[6] “Sensorineural deafness and seizures in mice lacking vesicular glutamate transporter 3.”  Seal R.P.et.al.   18215623

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPFKAFDTFK EKILKPGKEG VKNAVGDSLG ILQRKIDGTN EEEDAIELNE EGRPVQTSRA 
61:	HRPVCDCSCC GIPKRYICDC SCCGIPKRYI IAVMSGLGFC ISFGIRCNLG VAIVEMVNNS 
121:	TVYVDGKPEI QTAQFNWDPE TVGLIHGSFF WGYIVTQIPG GFISNKFAAS RVFGAAIFLT 
181:	STLNMFIPSA ARVHYGCVMG VRILQGLVEG VTYPACHGMW SKWAPPLERS RLATTSFCGS 
241:	YAGAVVAMPL AGVLVQYIGW ASVFYIYGMF GIIWYMFWLL QAYECPAAHP TISNAERTYI 
301:	ETSIGEGANL ASLSKFNTPW RRFFTSLPVY AIIVANFCRS WTFYLLLISQ PAYFEEVFGF 
361:	AISKVGLLSA VPHMVMTIVV PIGGQLADYL RSRKILTTTA VRKIMNCGGF GMEATLLLVV 
421:	GFSHTKGVAI SFLVLAVGFS GFAISGFNVN HLDIAPRYAS ILMGISNGVG TLSGMVCPLI 
481:	VGAMTKHKTR EEWQNVFLIA ALVHYSGVIF YGVFASGEKQ DWADPENLSE DKCGIIDQDE 
541:	LAEETELNHE TFVSPRKKMS YGATTQNCEV QKTEWRQQRE SAFDGEEPLS YQAEGDFSET 
601:	S