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2.A.1.19.14
Brush boarder glycosylated urate (Km= 1.2 mM) tranporter, RST. Orthologous to the human URAT1. Inhibited by 50 μM benzbromarone, 1 mM probenecid and 10 mM lactate which may also be transported and trans-stimulate urate uptake. May be orthologous to 2.A.1.19.11 as well (Hosoyamada et al., 2004).  Involved in urate absorption across the apical membrane, but probably not the primary route (Eraly et al. 2008; Prestin et al. 2014).

Accession Number:Q8CFZ5
Protein Name:Solute carrier family 22 (Organic anion/cation transporter), member 12 aka URAT1
Length:553
Molecular Weight:60194.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Lactate, Probenecid, Urate

Cross database links:

Genevestigator: Q8CFZ5
eggNOG: maNOG09069
HEGENOM: HBG713776
RefSeq: NP_033229.3   
Entrez Gene ID: 20521   
Pfam: PF00083   
KEGG: mmu:20521   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0031526 C:brush border membrane
GO:0016021 C:integral to membrane
GO:0030165 F:PDZ domain binding
GO:0015143 F:urate transmembrane transporter activity
GO:0042493 P:response to drug
GO:0015747 P:urate transport

References (3)

[1] “Kidney-specific expression of a novel mouse organic cation transporter-like protein.”  Mori K.et.al.   9409754
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “Function and localization of urate transporter 1 in mouse kidney.”  Hosoyamada M.et.al.   14747372

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAFPELLDRV GGLGRFQLFQ TVALVTPILW VTTQNMLENF SAAVPHHRCW VPLLDNSTSQ 
61:	ASIPGDLGPD VLLAVSIPPG PDQQPHQCLR FRQPQWQLTE SNATATNWSD AATEPCEDGW 
121:	VYDHSTFRST IVTTWDLVCN SQALRPMAQS IFLAGILVGA AVCGHASDRF GRRRVLTWSY 
181:	LLVSVSGTAA AFMPTFPLYC LFRFLLASAV AGVMMNTASL LMEWTSAQGS PLVMTLNALG 
241:	FSFGQVLTGS VAYGVRSWRM LQLAVSAPFF LFFVYSWWLP ESARWLITVG KLDQGLQELQ 
301:	RVAAVNRRKA EGDTLTMEVL RSAMEEEPSR DKAGASLGTL LHTPGLRHRT IISMLCWFAF 
361:	GFTFYGLALD LQALGSNIFL LQALIGIVDF PVKTGSLLLI SRLGRRFCQV SFLVLPGLCI 
421:	LSNILVPHGM GVLRSALAVL GLGCLGGAFT CITIFSSELF PTVIRMTAVG LCQVAARGGA 
481:	MLGPLVRLLG VYGSWMPLLV YGVVPVLSGL AALLLPETKN LPLPDTIQDI QKQSVKKVTH 
541:	DTPDGSILMS TRL