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2.A.1.19.34
Multispecific drug transporter, solute carrier family 22 member 8 (Organic anion transporter 3) (hOAT3).  Both OAT1 and OAT3 of humans are inhibited by caffeic acid (Ki ~ 17 μM) (Uwai et al. 2011; Wu et al. 2013).  It is the principal uptake system for steviol glucuronide (SVG), the major metabolite derived from steviol, the aglycone of stevioside and rebaudioside A (Wang et al. 2015).  Also functions in urate uptake from the circulation across the basolateral membrane of renal tubular cells (Prestin et al. 2014). See also 2.A.1.19.9.

Accession Number:Q8TCC7
Protein Name:Solute carrier family 22 member 8
Length:542
Molecular Weight:59856.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Basolateral cell membrane1 / Multi-pass membrane protein2
Substrate Steviol glucuronide

Cross database links:

Genevestigator: Q8TCC7
eggNOG: COG0477
HEGENOM: HOG000234569
Entrez Gene ID: 9376   
Pfam: PF00083   
Drugbank: Drugbank Link   
KEGG: hsa:9376   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0016021 C:integral to membrane
GO:0005452 F:inorganic anion exchanger activity
GO:0008514 F:organic anion transmembrane transporter activity
GO:0015651 F:quaternary ammonium group transmembrane transporter activity
GO:0034635 P:glutathione transport
GO:0031427 P:response to methotrexate
GO:0009636 P:response to toxin

References (9)

[1] “Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3).”  Race J.E.et.al.   10049739
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “Gene expression levels and immunolocalization of organic ion transporters in the human kidney.”  Motohashi H.et.al.   11912245
[4] “Human organic anion transporter 3 (hOAT3) can operate as an exchanger and mediate secretory urate flux.”  Bakhiya A.et.al.   14586168
[5] “Transport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8).”  Srimaroeng C.et.al.   15644426
[6] “Molecular cloning and functional analyses of OAT1 and OAT3 from cynomolgus monkey kidney.”  Tahara H.et.al.   15846473
[7] “Inhibition of oat3-mediated renal uptake as a mechanism for drug-drug interaction between fexofenadine and probenecid.”  Tahara H.et.al.   16455804
[8] “Polymorphisms of OATP-C (SLC21A6) and OAT3 (SLC22A8) genes: consequences for pravastatin pharmacokinetics.”  Nishizato Y.et.al.   12811365
[9] “The human organic anion transporter 3 (OAT3; SLC22A8): genetic variation and functional genomics.”  Erdman A.R.et.al.   16291576

External Searches:

  • Search: DB with
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  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTFSEILDRV GSMGHFQFLH VAILGLPILN MANHNLLQIF TAATPVHHCR PPHNASTGPW 
61:	VLPMGPNGKP ERCLRFVHPP NASLPNDTQR AMEPCLDGWV YNSTKDSIVT EWDLVCNSNK 
121:	LKEMAQSIFM AGILIGGLVL GDLSDRFGRR PILTCSYLLL AASGSGAAFS PTFPIYMVFR 
181:	FLCGFGISGI TLSTVILNVE WVPTRMRAIM STALGYCYTF GQFILPGLAY AIPQWRWLQL 
241:	TVSIPFFVFF LSSWWTPESI RWLVLSGKSS KALKILRRVA VFNGKKEEGE RLSLEELKLN 
301:	LQKEISLAKA KYTASDLFRI PMLRRMTFCL SLAWFATGFA YYSLAMGVEE FGVNLYILQI 
361:	IFGGVDVPAK FITILSLSYL GRHTTQAAAL LLAGGAILAL TFVPLDLQTV RTVLAVFGKG 
421:	CLSSSFSCLF LYTSELYPTV IRQTGMGVSN LWTRVGSMVS PLVKITGEVQ PFIPNIIYGI 
481:	TALLGGSAAL FLPETLNQPL PETIEDLENW SLRAKKPKQE PEVEKASQRI PLQPHGPGLG 
541:	SS