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2.A.1.19.4
The polyspecific organic anion, cation and neutral molecule transporter, Oat1 (Slc22a6) (transports neutral compounds such as cardiac glycosides [i.e., ouabain] and steroids [i.e., aldosterone; cortisol; dexamethasone]; cationic compounds such as N-propylajmalinium, and anionic compounds such as p-aminohippurate, dicarboxylates, cyclic nucleotides, prostaglandins, urate, β-lactam antibiotics, nonsteroidal anti-inflammatory drugs, diuretics, bile salts and steroid conjugates [i.e., estrone-3-sulfate and estradiol-17-glucuronide]) transporter (H+ symporter or uniporter) Probably catalyzes organic anion (uptake):dicarboxylate (efflux) antiport in the basolateral membrane of kidney proximal tubules) (Eraly et al., 2003a,b). A 3-dimensional model of OAT1 has led to the identification of residues involved in differential transport of substrates such as p-aminohippurate and cidofovir (Perry et al., 2006). Oat1 transports many antiviral agents (Truong et al., 2008).  The human orthologue (Q4U2R8; 563aas) has been shown to be a multispecific organic anion transporter on the basolateral membrane of the proximal tubule in human kidney (Hosoyamada et al. 1999). A substrate binding hinge domain is required for transport-related structural changes (Egenberger et al., 2012). Transports environmental toxins and clinically important drugs including anti-HIV therapeutics, anti-tumor drugs, antibiotics, anti-hypertensives, and anti-inflammatories (Duan et al., 2011). hOAT1 has two GXXXG motifs in TMSs 2 and 5 which play critical roles in stability (Duan et al., 2011).  Both OAT1 and OAT3 of humans are inhibited by caffeic acid (Ki ~ 17 μM) (Uwai et al. 2011).

Accession Number:O35956
Protein Name:Oat1 aka SLC22A6 aka ROAT1
Length:551
Molecular Weight:60766.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate steroids, N-propyl ajmalinium, Dicarboxylates, Cyclic nucleotides, Beta-lactam antibiotics, drugs, Diuretics, bile salts, Steroid conjugates, H+

Cross database links:

Genevestigator: O35956
RefSeq: NP_058920.1   
Entrez Gene ID: 29509   
Pfam: PF00083   
KEGG: rno:29509   

Gene Ontology

GO:0005901 C:caveola
GO:0016021 C:integral to membrane
GO:0005624 C:membrane fraction
GO:0043234 C:protein complex
GO:0005515 F:protein binding
GO:0015347 F:sodium-independent organic anion transmembr...
GO:0031427 P:response to methotrexate
GO:0043252 P:sodium-independent organic anion transport

References (7)

[1] “Expression cloning and characterization of ROAT1. The basolateral organic anion transporter in rat kidney.”  Sweet D.H.et.al.   9374486
[2] “Expression cloning and characterization of a novel multispecific organic anion transporter.”  Sekine T.et.al.   9228014
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “The antiviral nucleotide analogs cidofovir and adefovir are novel substrates for human and rat renal organic anion transporter 1.”  Cihlar T.et.al.   10462545
[5] “Molecular cloning and functional analyses of OAT1 and OAT3 from cynomolgus monkey kidney.”  Tahara H.et.al.   15846473
[6] “Prostaglandin E2 inhibits its own renal transport by downregulation of organic anion transporters rOAT1 and rOAT3.”  Sauvant C.et.al.   16338963
[7] “Downregulation of organic anion transporters in rat kidney under ischemia/reperfusion-induced acute renal failure.”  Matsuzaki T.et.al.   17245393

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAFNDLLKQV GGVGRFQLIQ VTMVVAPLLL MASHNTLQNF TAAIPPHHCR PPANANLSKD 
61:	GGLEAWLPLD KQGQPESCLR FTSPQWGPPF YNGTEANGTR VTEPCIDGWV YDNSTFPSTI 
121:	VTEWNLVCSH RAFRQLAQSL YMVGVLLGAM VFGYLADRLG RRKVLILNYL QTAVSGTCAA 
181:	YAPNYTVYCV FRLLSGMSLA SIAINCMTLN VEWMPIHTRA YVGTLIGYVY SLGQFLLAGI 
241:	AYAVPHWRHL QLVVSVPFFI AFIYSWFFIE SARWYSSSGR LDLTLRALQR VARINGKQEE 
301:	GAKLSIEVLR TSLQKELTLS KGQASAMELL RCPTLRHLFL CLSMLWFATS FAYYGLVMDL 
361:	QGFGVSMYLI QVIFGAVDLP AKFVCFLVIN SMGRRPAQMA SLLLAGICIL VNGIIPKSHT 
421:	IIRTSLAVLG KGCLASSFNC IFLYTGELYP TVIRQTGLGM GSTMARVGSI VSPLVSMTAE 
481:	FYPSMPLFIF GAVPVVASAV TALLPETLGQ PLPDTVQDLK SRSRGKQNQQ QQEQQKQMMP 
541:	LQASTQEKNG L