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2.A.1.19.7
The polyspecific organic anion (and cation) (anions: p-aminohippurate, ochratoxin A, estrone sulfate, anionic drugs, anionic neurotransmitter metabolites; cation: cimetidine) transporter, Oat3 (slc22a8) (catalyzes organic anion (uptake): dicarboxylate (efflux) antiport in the basolateral membrane of the renal proximal tubule) (Eraly et al., 2003a,b); transports many antiviral agents (Truong et al., 2008).

Accession Number:Q9R1U7
Protein Name:Oat3
Length:536
Molecular Weight:59186.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Basolateral cell membrane1 / Multi-pass membrane protein2
Substrate p-aminohippurate, Ochratoxin A, Estrone sulfate, Anionic neurotransmitter metabolites, Cimetidine, Dicarboxylates

Cross database links:

Genevestigator: Q9R1U7
eggNOG: roNOG07425
RefSeq: NP_112622.1   
Entrez Gene ID: 83500   
Pfam: PF00083   
KEGG: rno:83500   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0016021 C:integral to membrane
GO:0015651 F:quaternary ammonium group transmembrane tra...
GO:0015697 P:quaternary ammonium group transport
GO:0031427 P:response to methotrexate
GO:0009636 P:response to toxin
GO:0055085 P:transmembrane transport

References (11)

[1] “Molecular cloning and characterization of a new multispecific organic anion transporter from rat brain.”  Kusuhara H.et.al.   10224140
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “Expression and functional characterization of rat organic anion transporter 3 (rOat3) in the choroid plexus.”  Nagata Y.et.al.   11961115
[4] “Functional involvement of rat organic anion transporter 3 (rOat3; Slc22a8) in the renal uptake of organic anions.”  Hasegawa M.et.al.   11861777
[5] “Contribution of organic anion transporters to the renal uptake of anionic compounds and nucleoside derivatives in rat.”  Hasegawa M.et.al.   12660303
[6] “Contribution of organic anion transporter 3 (Slc22a8) to the elimination of p-aminohippuric acid and benzylpenicillin across the blood-brain barrier.”  Kikuchi R.et.al.   12684544
[7] “Organic anion transporter 3 (Slc22a8) is a dicarboxylate exchanger indirectly coupled to the Na+ gradient.”  Sweet D.H.et.al.   12488248
[8] “Involvement of multiple transporters in the efflux of 3-hydroxy-3-methylglutaryl-CoA reductase inhibitors across the blood-brain barrier.”  Kikuchi R.et.al.   15292460
[9] “Carrier-mediated uptake of H2-receptor antagonists by the rat choroid plexus: involvement of rat organic anion transporter 3.”  Nagata Y.et.al.   15319347
[10] “Involvement of rat organic anion transporter 3 in the uptake of an organic herbicide, 2,4-dichlorophenoxyacetate, by the isolated rat choroid plexus.”  Nagata Y.et.al.   15389674
[11] “Differential contributions of rOat1 (Slc22a6) and rOat3 (Slc22a8) to the in vivo renal uptake of uremic toxins in rats.”  Deguchi T.et.al.   15846470

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTFSEILDRV GSMGPFQYLH VTLLALPVLG IANHNLLQIF TATTPVHHCR PPPNASIGPW 
61:	VLPLDPNGKP EKCLRFVHLP NASLPNDTQR ATEPCLDGWI YNSTRDTIVI EWDLVCSSNK 
121:	LKEMAQSIFM AGILVGGPVI GELSDRFGRK PILTWSYLML AASGSGAAFS PSLPVYMIFR 
181:	FLCGCSISGI SLSTVILNVE WVPTSMRAIS STSIGYCYTI GQFILSGLAY AIPQWRWLQL 
241:	TSSAPFFIFS LLSWWVPESI RWLVLSGKYS KALKTLQRVA TFNGKKEEGK KLTIEELKFN 
301:	LQKDITSAKV KYGLSDLFRV SILRRVTFCL SLAWFSTGFA YYSLAMGVEE FGVNIYILQI 
361:	IFGGVDIPAK FITILSLSYL GRRITQSFLL LLAGGAILAL IFVPSEMQLL RTALAVFGKG 
421:	CLSGSFSCLF LYTSELYPTV LRQTGMGISN VWARVGSMIA PLVKITGELQ PFIPNVIFGT 
481:	TALLGGSAAF FLLETLNRPL PETIEDIQNW HKQVQKTKQE SEAEKASQII PLKTGG