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2.A.1.19.9
The osteosclerosis protein, Roct (organic anion transporter 3, Oat3) (Slc22a8) (catalyzes organic anion (uptake):di-carboxylate (efflux) antiport in the basolateral membrane of the renal proximal tubule) (Eraly et al., 2003a,b); transports glutathione and many antiviral agents (Truong et al., 2008).  It is a multispecific drug transporter, critical for the renal handling of common drugs (e.g, antibiotics, antivirals, diuretics) and toxins.  Probably handles hydroxylated and glucouronidated metabolites, consistent with the "remote sensing and signaling hypothesis" (Wu et al. 2013).  It may also handle dietary flavonoids and antioxidants.

Accession Number:O88909
Protein Name:Roct aka Oat3 aka SLC22A8
Length:537
Molecular Weight:59246.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Basolateral cell membrane1 / Multi-pass membrane protein2
Substrate Dicarboxylates, Glutathione, Antivirals, Glucouronidated metabolites, Flavonoids, drugs

Cross database links:

Genevestigator: O88909
eggNOG: roNOG07425
HEGENOM: HBG713776
RefSeq: NP_001158106.1    NP_001158107.1    NP_112471.3   
Entrez Gene ID: 19879   
Pfam: PF00083   
KEGG: mmu:19879   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0016021 C:integral to membrane
GO:0009636 P:response to toxin

References (6)

[1] “A novel putative transporter maps to the osteosclerosis (oc) mutation and is not expressed in the oc mutant mouse.”  Brady K.P.et.al.   10087192
[2] “The transcriptional landscape of the mammalian genome.”  Carninci P.et.al.   16141072
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Impaired organic anion transport in kidney and choroid plexus of organic anion transporter 3 (Oat3 (Slc22a8)) knockout mice.”  Sweet D.H.et.al.   12011098
[5] “Organic anion transport in choroid plexus from wild-type and organic anion transporter 3 (Slc22a8)-null mice.”  Sykes D.et.al.   15075193
[6] “Renal transport of organic compounds mediated by mouse organic anion transporter 3 (mOat3): further substrate specificity of mOat3.”  Kobayashi Y.et.al.   15100168

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTFSEILDRV GSMGPFQYLH VTLLALPILG IANHNLLQIF TATTPDHHCR PPPNASLEPW 
61:	VLPLGPNGKP EKCLRFVHLP NASLPNDTQG ATEPCLDGWI YNSTRDTIVT EWDLVCGSNK 
121:	LKEMAQSVFM AGILVGGPVF GELSDRFGRK PILTWSYLLL AASGSSAAFS PSLTVYMIFR 
181:	FLCGCSISGI SLSTIILNVE WVPTSTRAIS STTIGYCYTI GQFILPGLAY AVPQWRWLQL 
241:	SVSAAFFIFS LLSWWVPESI RWLVLSGKFS KALKTLQRVA TFNGKKEEGE KLTVEELKFN 
301:	LQKDITSAKV KYGLSDLFRV SILRRVTFCL SLAWFATGFA YYSLAMGVEE FGVNIYILQI 
361:	IFGGVDIPAK FITILSISYL GRRITQGFLL ILAGVAILAL IFVSSEMQLL RTALAVFGKG 
421:	CLSGSFSCLF LYTSELYPTV LRQTGMGISN IWARVGSMIA PLVKITGELQ PFIPNVIFGT 
481:	MTLLGGSAAF FLLETLNRPL PETIEDIQDW YQQTKKTKQE PEAEKASQTI PLKTGGP