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2.A.1.2.1
Pyridoxine, pyridoxal, pyridoxamine, amiloride:H+ cotransporter (Km (pyridoxine) = 22 μM) (Stolz et al., 2005). Also takes up thiamine (Vogl et al., 2008).

Accession Number:P33532
Protein Name:Car1 aka SOD1 aka SPAC17A2.01 aka SPAC1B1.05
Length:526
Molecular Weight:58484.00
Species:Schizosaccharomyces pombe (Fission yeast) [4896]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Pyridoxine, Amiloride, H+, Thiamine, Pyridoxal

Cross database links:

eggNOG: KOG0255 KOG0255 KOG0255
HEGENOM: HBG676894 HBG676894 HBG676894
RefSeq: XP_001713084.1   
Entrez Gene ID: 3361460   
Pfam: PF07690   
KEGG: spo:SPAC17A2.01    spo:SPAC17A2.01    spo:SPAC17A2.01   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0015238 F:drug transmembrane transporter activity
GO:0031925 F:pyridoxal transmembrane transporter activity
GO:0031927 F:pyridoxamine transmembrane transporter acti...
GO:0031928 F:pyridoxine transmembrane transporter activity
GO:0015234 F:thiamin transmembrane transporter activity
GO:0015893 P:drug transport
GO:0031920 P:pyridoxal transport
GO:0031922 P:pyridoxamine transport
GO:0031923 P:pyridoxine transport
GO:0015888 P:thiamin transport
GO:0031927 F:pyridoxamine transmembrane transporter activity
GO:0015234 F:thiamine transmembrane transporter activity

References (9)

[1] “The amiloride resistance gene, car1, of Schizosaccharomyces pombe.”  Jia Z.-P.et.al.   8246883
[2] “The genome sequence of Schizosaccharomyces pombe.”  Wood V.et.al.   11859360
[3] “Amiloride uptake and toxicity in fission yeast are caused by the pyridoxine transporter encoded by bsu1+ (car1+).”  Stolz J.et.al.   15701794
[4] “The amiloride resistance gene, car1, of Schizosaccharomyces pombe.”  Jia Z.-P.et.al.   8246883
[5] “The genome sequence of Schizosaccharomyces pombe.”  Wood V.et.al.   11859360
[6] “Amiloride uptake and toxicity in fission yeast are caused by the pyridoxine transporter encoded by bsu1+ (car1+).”  Stolz J.et.al.   15701794
[7] “The amiloride resistance gene, car1, of Schizosaccharomyces pombe.”  Jia Z.-P.et.al.   8246883
[8] “The genome sequence of Schizosaccharomyces pombe.”  Wood V.et.al.   11859360
[9] “Amiloride uptake and toxicity in fission yeast are caused by the pyridoxine transporter encoded by bsu1+ (car1+).”  Stolz J.et.al.   15701794

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MASKIASLFS PSETASKDQH ENVAEDLELG TASSQSDGIH ETNSEYDEKK REESPEVIDI 
61:	SNLISSDHPA HPQNWHWAKR WSIVFMFCLM QIYVIWTSNG FGSIEYSVMA QFNVSAQVAT 
121:	LCLSMNILGS GLGPMFLGPL SDIGGRKPVY FCSIFVYTVF NISCALPRNI VQMIISHFII 
181:	GVAGSTALTN VAGGIPDLFP EDTAGVPMSL FVWACAGGAI GAPMATGVDI NAKYGWRWLY 
241:	YINIIVGGFF LIVILIIPET LPIKVITRYE NAKGRIVEGI PKNNLKEVLK KCKFVTTMGF 
301:	RMMLTEPIIL SMGLYNFYAY GISYFFLTAI WPVFYDTYKM SEMGASCTYL SGFVASTLLF 
361:	LYQPIQDWIF RRDKAKNNGV ARPEARFTSA LFITLLFPAG MFLFAFTCHP PFPWMSPIVG 
421:	NSMVTVANGH NWMCILNYLT DSYPLLSGSA VAAFTLPSFI GATVFAHVSQ IMFNNMSVKW 
481:	AVATMAFISI SIPFIIYTFY FFGQRIRALS SLTGNKALKY LPLENN