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2.A.1.2.16
Polyamines (spermine, spermidine, putrescine); paraquat; methylglyoxal bis(guanylhydrazone):H+ antiporter (in the plasma membrane) (activated by phosphorylation) (Uemura et al., 2005)

Accession Number:Q07824
Protein Name:TPO1 aka YLL028w
Length:586
Molecular Weight:64272.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Spermine, Spermidine, Putrescine, Paraquat, Methylglyoxal bis(guanylhydrazone), H+

Cross database links:

Genevestigator: Q07824
eggNOG: fuNOG06560
HEGENOM: HBG676874
DIP: DIP-1706N
RefSeq: NP_013072.1   
Entrez Gene ID: 850631   
Pfam: PF07690   
KEGG: sce:YLL028W   

Gene Ontology

GO:0033101 C:cellular bud membrane
GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0015297 F:antiporter activity
GO:0015238 F:drug transmembrane transporter activity
GO:0042802 F:identical protein binding
GO:0015606 F:spermidine transmembrane transporter activity
GO:0000297 F:spermine transmembrane transporter activity
GO:0015893 P:drug transport
GO:0015847 P:putrescine transport
GO:0015848 P:spermidine transport
GO:0000296 P:spermine transport
GO:0055085 P:transmembrane transport

References (16)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.”  Johnston M.et.al.   9169871
[2] “Identification of a gene for a polyamine transport protein in yeast.”  Tomitori H.et.al.   9920864
[3] “Multiple polyamine transport systems on the vacuolar membrane in yeast.”  Tomitori H.et.al.   11171066
[4] “Novel target genes of the yeast regulator Pdr1p: a contribution of the TPO1 gene in resistance to quinidine and other drugs.”  do Valle Matta M.A.et.al.   11470516
[5] “Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae.”  Ficarro S.B.et.al.   11875433
[6] “Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions.”  Kim H.et.al.   12524434
[7] “Localization and function of the yeast multidrug transporter Tpo1p.”  Albertsen M.et.al.   12562762
[8] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[9] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[10] “Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis.”  Shepard K.A.et.al.   13679573
[11] “Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively.”  Aranda A.et.al.   15066780
[12] “Characteristics of the polyamine transporter TPO1 and regulation of its activity and cellular localization by phosphorylation.”  Uemura T.et.al.   15637075
[13] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[14] “Identification of the TPO1 gene in yeast, and its human orthologue TETRAN, which cause resistance to NSAIDs.”  Mima S.et.al.   17362938
[15] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[16] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSDHSPISNK ENHLLPSDSS RSSSSDMHST GTTGTTGVEP VDFTGEGAKY TTATEGNGGA 
61:	DLAIQRTTTM NSAAESEVNI TRRLTKILTG SVNEPDRVEV DYTNCAPMGG DRPYPPSLPS 
121:	RDLYEVTFDG PNDPLHPFNW PMKKKVLLCL VLCLDSIAIA MCSSIFASAV PQICEIYHVI 
181:	EVVAILGITL FVLGFAASPV IYAPLSELYG RKGVLVLSAF GFALFQFAVA TAENLQTIFI 
241:	CRFFGGFIGA APMAVVPAAF ADMFDTNVRG KAIALFSLGV FVGPILSPVM GSYIAQRTTW 
301:	RWLEYVVGCF ASAVFVAIVL FFEETHHPTI LVNKAKQMRK QSNNWGIHAA HEDVELSIKD 
361:	IVQKTVTRPI IMLFVEPLLL FVTIYNSFVY GILYLLLEAY PLVFVEGYGF TENGELPYIA 
421:	LIIGMMVCAA FIWYMDNDYL KRCRAKGKLV PEARLYAMVI AGTVFPIGIL WFCWTGYYPH 
481:	KIHWMVPTVG GAFIGFGLMG IFLPCLNYII ESYLLLAASA VAANTFMRSA FGACFPLFAG 
541:	YMFRGMGIGW AGLLLGLFAA AMIPVPLLFL KYGESIRKKS KYAYAA