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2.A.1.2.20
Broad specificity MDR efflux pump, MdtG (YceE) (under SoxSR control) (Fàbrega et al., 2010).  Confer resistance to fosfomycin, fluoroquinolone and many other drugs (Nishino and Yamaguchi 2001).

Accession Number:P25744
Protein Name:YceE aka B1053
Length:408
Molecular Weight:43867.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate fosfomycin, Fluoroquinolones

Cross database links:

Genevestigator: P25744
EchoBASE: EB1319
EcoGene: EG11343
eggNOG: COG0477
HEGENOM: HBG404070
RefSeq: AP_001679.1    NP_415571.1   
Entrez Gene ID: 945627   
Pfam: PF07690   
BioCyc: EcoCyc:YCEE-MONOMER    ECOL168927:B1053-MONOMER   
KEGG: ecj:JW1040    eco:b1053   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015520 F:tetracycline:hydrogen antiporter activity
GO:0046677 P:response to antibiotic
GO:0015904 P:tetracycline transport
GO:0055085 P:transmembrane transport

References (5)

[1] “Multicopy suppression: an approach to understanding intracellular functioning of the protein export system.”  Ueguchi C.et.al.   1537791
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Analysis of a complete library of putative drug transporter genes in Escherichia coli.”  Nishino K.et.al.   11566977

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSPCENDTPI NWKRNLIVAW LGCFLTGAAF SLVMPFLPLY VEQLGVTGHS ALNMWSGIVF 
61:	SITFLFSAIA SPFWGGLADR KGRKLMLLRS ALGMGIVMVL MGLAQNIWQF LILRALLGLL 
121:	GGFVPNANAL IATQVPRNKS GWALGTLSTG GVSGALLGPM AGGLLADSYG LRPVFFITAS 
181:	VLILCFFVTL FCIREKFQPV SKKEMLHMRE VVTSLKNPKL VLSLFVTTLI IQVATGSIAP 
241:	ILTLYVRELA GNVSNVAFIS GMIASVPGVA ALLSAPRLGK LGDRIGPEKI LITALIFSVL 
301:	LLIPMSYVQT PLQLGILRFL LGAADGALLP AVQTLLVYNS SNQIAGRIFS YNQSFRDIGN 
361:	VTGPLMGAAI SANYGFRAVF LVTAGVVLFN AVYSWNSLRR RRIPQVSN