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2.A.1.2.25
The purine base/nucleoside (nucleosides: inosine, adenosine and guanosine; bases: hypoxanthine, adenine, guanine, 2-fluoroadenine) efflux pump, YdhL (PbuE) (Johansen et al., 2003; Nygaard and Saxild, 2005; Zakataeva et al., 2007; Sheremet et al. 2011Sheremet et al. 2011).

Accession Number:Q797E3
Protein Name:Purine efflux pump pbuE
Length:388
Molecular Weight:41535.00
Species: [1423]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Inosine, Adenosine, Guanosine, Adenine, 2-fluoroadenine, guanine, Hypoxanthine

Cross database links:

HEGENOM: HBG732715
RefSeq: NP_388461.2   
Entrez Gene ID: 937996   
Pfam: PF07690   
KEGG: bsu:BSU05800   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0055085 P:transmembrane transport

References (6)

[1] “Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome.”  Sadaie Y.et.al.   9202461
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[3] “Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).”  Johansen L.E.et.al.   12923093
[4] “Adenine riboswitches and gene activation by disruption of a transcription terminator.”  Mandal M.et.al.   14718920
[5] “The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size.”  Nygaard P.et.al.   15629952
[6] “A new function for the Bacillus PbuE purine base efflux pump: efflux of purine nucleosides.”  Zakataeva N.P.et.al.   17935948

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNFKVFLLAA STIAVGLVEL IVGGILPQIA NDLDISIVSA GQLISVFALG YAVSGPLLLA 
61:	LTAKIERKRL YLIALFVFFL SNLVAYFSPN FATLMVSRVL AAMSTGLIVV LSLTIAPKIV 
121:	APEYRARAIG IIFMGFSSAI ALGVPLGILI SDSFGWRILF LGIGLLALIS MLIISIFFER 
181:	IPAEKMIPFR EQLKTIGNLK IASSHLVTMF TLAGHYTLYA YFAPFLEETL HLSSFWVSIC 
241:	YFLFGISAVC GGPFGGALSD RLGSFKSILL VTGSFAIIMF LLPLSTSSMI FFLPVMVIWG 
301:	LLSWSLAPAQ QSYLIEIAPD SSDIQQSFNT SALQVGIALG SAIGGVVLDQ TGTVVSTAWC 
361:	GGSIVIIAVL FAFISLTRPV QTAKKSSL