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2.A.1.2.26
The purine ribonucleoside (inosine, adenosine, guanosine, 6-mercaptopurine ribonucleoside) efflux pump (H+ antiporter), NepI (YicM) (Gronskiy et al., 2005; Sheremet et al. 2011)

Accession Number:P0ADL1
Protein Name:Purine ribonucleoside efflux pump NepI (aka YicM)
Length:396
Molecular Weight:41842.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate purine ribonucleosides, 6-mercaptopurine ribonucleoside, Purine

Cross database links:

Genevestigator: P0ADL1
EchoBASE: EB1640
EcoGene: EG11689
eggNOG: COG2814
HEGENOM: HBG732715
DIP: DIP-48180N
RefSeq: AP_004130.1    NP_418118.2   
Entrez Gene ID: 948213   
Pfam: PF07690   
BioCyc: EcoCyc:YICM-MONOMER    ECOL168927:B3662-MONOMER   
KEGG: ecj:JW5938    eco:b3662   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015211 F:purine nucleoside transmembrane transporter...
GO:0055085 P:transmembrane transport

References (6)

[1] “DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.”  Burland V.D.et.al.   7686882
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “A selC-associated genomic island of the extraintestinal avian pathogenic Escherichia coli strain BEN2908 is involved in carbohydrate uptake and virulence.”  Chouikha I.et.al.   16428402
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[6] “The yicM (nepI) gene of Escherichia coli encodes a major facilitator superfamily protein involved in efflux of purine ribonucleosides.”  Gronskiy S.V.et.al.   16040204

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSEFIAENRG ADAITRPNWS AVFSVAFCVA CLIIVEFLPV SLLTPMAQDL GISEGVAGQS 
61:	VTVTAFVAMF ASLFITQTIQ ATDRRYVVIL FAVLLTLSCL LVSFANSFSL LLIGRACLGL 
121:	ALGGFWAMSA SLTMRLVPPR TVPKALSVIF GAVSIALVIA APLGSFLGEL IGWRNVFNAA 
181:	AVMGVLCIFW IIKSLPSLPG EPSHQKQNTF RLLQRPGVMA GMIAIFMSFA GQFAFFTYIR 
241:	PVYMNLAGFG VDGLTLVLLS FGIASFIGTS LSSFILKRSV KLALAGAPLI LAVSALVLTL 
301:	WGSDKIVATG VAIIWGLTFA LVPVGWSTWI TRSLADQAEK AGSIQVAVIQ LANTCGAAIG 
361:	GYALDNIGLT SPLMLSGTLM LLTALLVTAK VKMKKS