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2.A.1.2.28
The vesicular acetylcholine transporter, VAChT (pumps acetylcholine into synaptic vesicles).  The acetyl choline and vesamicol binding sites are near the luminal end of the transport pathway (Khare et al. 2010).

Accession Number:Q16572
Protein Name:VAChT
Length:532
Molecular Weight:56961.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Acetylcholine

Cross database links:

Genevestigator: Q16572 Q16572
eggNOG: maNOG17267 NOG255549
HEGENOM: HBG714968 HOG000018545
RefSeq: NP_003046.2   
Entrez Gene ID: 6572   
Pfam: PF07690   
OMIM: 600336  gene
KEGG: hsa:6572    hsa:6572   

Gene Ontology

GO:0060201 C:clathrin sculpted acetylcholine transport v...
GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0005277 F:acetylcholine transmembrane transporter act...
GO:0015870 P:acetylcholine transport
GO:0055085 P:transmembrane transport
GO:0030121 C:AP-1 adaptor complex
GO:0030122 C:AP-2 adaptor complex
GO:0043679 C:axon terminus
GO:0060201 C:clathrin sculpted acetylcholine transport vesicle membrane
GO:0008021 C:synaptic vesicle
GO:0042166 F:acetylcholine binding
GO:0005277 F:acetylcholine transmembrane transporter activity
GO:0007269 P:neurotransmitter secretion

References (7)

[1] “Functional identification of a vesicular acetylcholine transporter and its expression from a 'cholinergic' gene locus.”  Erickson J.D.et.al.   8071310
[2] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Functional identification of a vesicular acetylcholine transporter and its expression from a 'cholinergic' gene locus.”  Erickson J.D.et.al.   8071310
[5] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[6] “The DNA sequence and comparative analysis of human chromosome 10.”  Deloukas P.et.al.   15164054
[7] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MESAEPAGQA RAAATKLSEA VGAALQEPRR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY 
61:	IAHMRGGGEG PTRTPEVWEP TLPLPTPANA SAYTANTSAS PTAAWPAGSA LRPRYPTESE 
121:	DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVLFA FAEDYATLFA 
181:	ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG 
241:	KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT 
301:	CNIPLAFLEP TIATWMKHTM AASEWEMGMA WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG 
361:	ALGLAVIGAS SCIVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV 
421:	YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS 
481:	ERDVLLDEPP QGLYDAVRLR ERPVSGQDGE PRSPPGPFDE CEDDYNYYYT RS