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2.A.1.2.3
Chloramphenicol:H+ antiporter, CmlA; Cmr; MdfA.  Multidrug exporter that also catalyzes efflux of arabinose (but not xylose) and  isopropyl β-thiogalactoside (Koita and Rao 2012).

Accession Number:P32482
Protein Name:CmlA
Length:419
Molecular Weight:44316.00
Species:Pseudomonas aeruginosa [287]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Chloramphenicol, H+, Arabinose

Cross database links:

Pfam: PF07690   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015520 F:tetracycline:hydrogen antiporter activity
GO:0046677 P:response to antibiotic
GO:0015904 P:tetracycline transport
GO:0055085 P:transmembrane transport

References (2)

[1] “Characterization of the nonenzymatic chloramphenicol resistance (cmlA) gene of the In4 integron of Tn1696: similarity of the product to transmembrane transport proteins.”  Bissonnette L.et.al.   1648560
[2] “Sequence analysis of the inducible chloramphenicol resistance determinant in the Tn1696 integron suggests regulation by translational attenuation.”  Stokes H.W.et.al.   1658833

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSKNFSWRY SLAATVLLLS PFDLLASLGM DMYLPAVPFM PNALGTTAST IQLTLTTYLV 
61:	MIGAGQLLFG PLSDRLGRRP VLLGGGLAYV VASMGLALTS SAEVFLGLRI LQACGASACL 
121:	VSTFATVRDI YAGREESNVI YGILGSMLAM VPAVGPLLGA LVDMWLGWRA IFAFLGLGMI 
181:	AASAAAWRFW PETRVQRVAG LQWSQLLLPV KCLNFWLYTL CYAAGMGSFF VFFSIAPGLM 
241:	MGRQGVSQLG FSLLFATVAI AMVFTARFMG RVIPKWGSPS VLRMGMGCLI AGAVLLAITE 
301:	IWALQSVLGF IAPMWLVGIG VATAVSVAPN GALRGFDHVA GTVTAVYFCL GGVLLGSIGT 
361:	LIISLLPRNT AWPVVVYCLT LATVVLGLSC VSRVKGSRGQ GEHDVVALQS AESTSNPNR