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2.A.1.2.33
The Hol1 MFS transporter (Mutation allows the uptake of histidinol and other cations (Wright et al., 1996). The N-terminal 200 residues show 22% identity with 2.A.1.2.1 and 2.A.1.2.16).

Accession Number:P53389
Protein Name:Protein Hol1
Length:586
Molecular Weight:65348.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Unknown

Cross database links:

Genevestigator: P53389
eggNOG: fuNOG06359
HEGENOM: HBG395394
DIP: DIP-7266N
RefSeq: NP_014453.1   
Entrez Gene ID: 855792   
Pfam: PF07690   
KEGG: sce:YNR055C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005739 C:mitochondrion
GO:0015665 F:alcohol transmembrane transporter activity
GO:0022890 F:inorganic cation transmembrane transporter ...
GO:0006812 P:cation transport
GO:0015850 P:organic alcohol transport
GO:0055085 P:transmembrane transport

References (3)

[1] “Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae.”  Wright M.B.et.al.   8955402
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.”  Philippsen P.et.al.   9169873
[3] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDKYTNRDHP DYIPGTFNIY SSQNLENGII YESKLKKTSS GVVLIPQPSY SPNDPLNWSS 
61:	WRKLAHFGLM AFITAFTAAT SNDAGAAQDS LNEIYGISYD SMNTGAGVLF LGIGWSTLFL 
121:	APFANLYGRK ITYIVCTTLG LFGALWFALA KRTSDTIWSQ LFVGISESCA EAQVQLSLSD 
181:	IFFQHQLGSV LTVYIMCTSI GTFLGPLIAG YISAFTNFRW VGWVAVIISG GLLITIIFGC 
241:	EETYFDRGQY MTPLTSCQSG YEDGTTLQNS DNTAVSRRKR HLDAKLSTPG AMGEKGVDLS 
301:	ETAEFEVNNE EEVTIPETRE LIDGSKEHLK PYPKRVAILT KATNLKGYGF KQYFKYLKIN 
361:	LRMFLFPPVW LSGMFWGIQD VFLTFYLTTQ ESAYYEPPWN YSDFGVAIMN VPTLIGAVIG 
421:	CICAGIVSDY FVLWMARHNR GILEAEFRLY FSIATAIIGP AGLLMFGIGT ARQWPWQAIY 
481:	VGLGFVGFAW GCSGDIAMAY LMDCYPDMVL EGMVCTAIIN NTISCIFTFT CSDWLAASGT 
541:	ENTYIALAVI NFGITAFALP MYYYGKRIRL WTKRWYLQSV NLRDGV