TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.1.2.52
Puromycin resistance MDR protein, MdtM (Soo et al., 2011).  Also catalyzes bile salt:H+ antiport, and binds cholate and deoxycholate to the protein with micromolar affinity. Functions as an MDR pump (Nishino and Yamaguchi 2001). Acts synergistically with AcrAB-TolC (Paul et al. 2014).

Accession Number:P39386
Protein Name:Multidrug resistance protein MdtM
Length:410
Molecular Weight:44688.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate puromycin, bile salts

Cross database links:

Genevestigator: P39386
EchoBASE: EB2464
EcoGene: EG12576
eggNOG: COG0477
HEGENOM: HBG289858
DIP: DIP-12641N
Entrez Gene ID: 948861   
Pfam: PF07690   
KEGG: ecj:JW4300    eco:b4337   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0046677 P:response to antibiotic
GO:0055085 P:transmembrane transport

References (5)

[1] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Analysis of a complete library of putative drug transporter genes in Escherichia coli.”  Nishino K.et.al.   11566977
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MPRFFTRHAA TLFFPMALIL YDFAAYLSTD LIQPGIINVV RDFNADVSLA PAAVSLYLAG 
61:	GMALQWLLGP LSDRIGRRPV LITGALIFTL ACAATMFTTS MTQFLIARAI QGTSICFIAT 
121:	VGYVTVQEAF GQTKGIKLMA IITSIVLIAP IIGPLSGAAL MHFMHWKVLF AIIAVMGFIS 
181:	FVGLLLAMPE TVKRGAVPFS AKSVLRDFRN VFCNRLFLFG AATISLSYIP MMSWVAVSPV 
241:	ILIDAGSLTT SQFAWTQVPV FGAVIVANAI VARFVKDPTE PRFIWRAVPI QLVGLSLLIV 
301:	GNLLSPHVWL WSVLGTSLYA FGIGLIFPTL FRFTLFSNKL PKGTVSASLN MVILMVMSVS 
361:	VEIGRWLWFN GGRLPFHLLA VVAGVIVVFT LAGLLNRVRQ HQAAELVEEQ