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2.A.1.2.64
Polyamine exporter 4 (Igarashi and Kashiwagi 2010).

Accession Number:Q12256
Protein Name:Polyamine transporter 4
Length:659
Molecular Weight:73223.00
Species:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Polyamines

Cross database links:

Genevestigator: Q12256
eggNOG: COG0477
HEGENOM: HOG000160689
DIP: DIP-4096N
Entrez Gene ID: 854447   
Pfam: PF07690   
KEGG: sce:YOR273C   

Gene Ontology

GO:0000329 C:fungal-type vacuole membrane
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0015606 F:spermidine transmembrane transporter activity
GO:0000297 F:spermine transmembrane transporter activity

References (13)

[1] “DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae.”  Cheret G.et.al.   8896271
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.”  Dujon B.et.al.   9169874
[3] “Identification of Saccharomyces cerevisiae genes conferring resistance to quinoline ring-containing antimalarial drugs.”  Delling U.et.al.   9593122
[4] “Multiple polyamine transport systems on the vacuolar membrane in yeast.”  Tomitori H.et.al.   11171066
[5] “Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae.”  Ficarro S.B.et.al.   11875433
[6] “Localization and function of the yeast multidrug transporter Tpo1p.”  Albertsen M.et.al.   12562762
[7] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[8] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[9] “Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively.”  Aranda A.et.al.   15066780
[10] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[11] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[12] “Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.”  Chi A.et.al.   17287358
[13] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPSSLTKTES NSDPRTNIQQ VPKALDKNVT NSGNLDSTSS STGSITEDEK RSEPNADSNN 
61:	MTGGEPIDPR DLDWDGPDDP DNPHNWSSLK KWYTTMTSAF LCLVVTMGSS LYVSSVPELV 
121:	ERYHVSQTLA LAGLTFYLLG LSTVIGAPLS EVFGRKPVYL FSLPVSMLFT MGVGLSNGHM 
181:	RIILPLRFLS GVFASPALSV GSGTILDIFD VDQVSVAMTY FVLSPFLGPV LSPIMAGFAT 
241:	EAKGWRWSEW IQLIAGGLIL PFIALMPETH KGIILRKRAK KRNIALKKFS REAQKEFLKT 
301:	TVTITILRPL KMLVVEPIVF VFSVYVAFIF AILFGFFEAY AVIYRGVYHM SMGISGLPFI 
361:	GIGVGLWIGA FFYLYIDRKY LFPKPPAGTQ PLTEKERTSK RTTPYRGARD AETGELLPVV 
421:	PEKFLIACKF GSVALPIGLF WQAWTARSDV HWMAPVAAGV PFGFGLILIF FSVLMYFSTC 
481:	YPPLTVASCL AANNLLRYVM SSVFPLFTIQ MYTKMKIKWA STLFALVCVV MIPIPWVFEK 
541:	WGSKLRHKSQ FGYAAMEKEA ETEGGIDDVN AVDGELNLTR MTTLRTMETD PSTREKPGER 
601:	LSLRRTHTQP VPASFDREDG QHAQNRNEPI SNSLYSAIKD NEDGYSYTEM ATDASARMV