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2.A.1.2.68
Tetracycline resistance protein, class B (TetA(B)) (Metal-tetracycline/H+ antiporter).  Mutants defective in either transport or tetracycline binding have been isolated (Wright and Tate 2015).

Accession Number:P02980
Protein Name:Tetracycline resistance protein, class B
Length:401
Molecular Weight:43267.00
Species:Escherichia coli [562]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate tetracycline

Cross database links:

DIP: DIP-16933N
Entrez Gene ID: 4924775   
Pfam: PF07690   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0046677 P:response to antibiotic

References (5)

[1] “Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322.”  Nguyen T.T.et.al.   6319234
[2] “Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.”  Hillen W.et.al.   6298728
[3] “Metal-tetracycline/H+ antiporter of Escherichia coli encoded by a transposon Tn10. Histidine 257 plays an essential role in H+ translocation.”  Yamaguchi A.et.al.   1848846
[4] “Metal-tetracycline/H+ antiporter of Escherichia coli encoded by a transposon, Tn10. The role of the conserved dipeptide, Ser65-Asp66, in tetracycline transport.”  Yamaguchi A.et.al.   2168416
[5] “Membrane topology of the transposon 10-encoded metal-tetracycline/H+ antiporter as studied by site-directed chemical labeling.”  Kimura T.et.al.   8995300

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MNSSTKIALV ITLLDAMGIG LIMPVLPTLL REFIASEDIA NHFGVLLALY ALMQVIFAPW 
61:	LGKMSDRFGR RPVLLLSLIG ASLDYLLLAF SSALWMLYLG RLLSGITGAT GAVAASVIAD 
121:	TTSASQRVKW FGWLGASFGL GLIAGPIIGG FAGEISPHSP FFIAALLNIV TFLVVMFWFR 
181:	ETKNTRDNTD TEVGVETQSN SVYITLFKTM PILLIIYFSA QLIGQIPATV WVLFTENRFG 
241:	WNSMMVGFSL AGLGLLHSVF QAFVAGRIAT KWGEKTAVLL GFIADSSAFA FLAFISEGWL 
301:	VFPVLILLAG GGIALPALQG VMSIQTKSHQ QGALQGLLVS LTNATGVIGP LLFAVIYNHS 
361:	LPIWDGWIWI IGLAFYCIII LLSMTFMLTP QAQGSKQETS A