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2.A.1.2.7
Bicyclomycin, sulfathiazole, tetracycline, fosfomycin, acriflavin, etc.):H+ antiporter (Nishino and Yamaguchi 2001).  Also exports L-cysteine (Yamada et al., 2006).

Accession Number:P28246
Protein Name:Bcr aka BICA aka BICR aka SUR aka SUXA aka B2182
Length:396
Molecular Weight:43353.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate bicyclomycin, sulfathiazole, tetracycline, fosfomycin, acriflavin, L-cysteine

Cross database links:

Genevestigator: P28246
EchoBASE: EB1390
EcoGene: EG11419
eggNOG: COG0477
HEGENOM: HBG300735
RefSeq: AP_002780.1    NP_416687.1   
Entrez Gene ID: 944808   
Pfam: PF07690   
BioCyc: EcoCyc:BCR-MONOMER    ECOL168927:B2182-MONOMER   
KEGG: ecj:JW5363    eco:b2182   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015238 F:drug transmembrane transporter activity
GO:0015893 P:drug transport
GO:0046677 P:response to antibiotic
GO:0055085 P:transmembrane transport

References (5)

[1] “Cloning and sequence analysis of an Escherichia coli gene conferring bicyclomycin resistance.”  Bentley J.et.al.   8486276
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Gene amplification contributes to sulfonamide resistance in Escherichia coli.”  Nichols B.P.et.al.   2694948
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MTTRQHSSFA IVFILGLLAM LMPLSIDMYL PALPVISAQF GVPAGSTQMT LSTYILGFAL 
61:	GQLIYGPMAD SFGRKPVVLG GTLVFAAAAV ACALANTIDQ LIVMRFFHGL AAAAASVVIN 
121:	ALMRDIYPKE EFSRMMSFVM LVTTIAPLMA PIVGGWVLVW LSWHYIFWIL ALAAILASAM 
181:	IFFLIKETLP PERRQPFHIR TTIGNFAALF RHKRVLSYML ASGFSFAGMF SFLSAGPFVY 
241:	IEINHVAPEN FGYYFALNIV FLFVMTIFNS RFVRRIGALN MFRSGLWIQF IMAAWMVISA 
301:	LLGLGFWSLV VGVAAFVGCV SMVSSNAMAV ILDEFPHMAG TASSLAGTFR FGIGAIVGAL 
361:	LSLATFNSAW PMIWSIAFCA TSSILFCLYA SRPKKR