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2.A.1.2.9
(Hydrophobic uncoupler e.g., CCCP, benzalkonium, SDS and other drugs):H+ antiporter, EmrD (Nishino and Yamaguchi 2001). The 3-d structure (3.5 Å resolution) has been determined (Yin et al., 2006).  conformational dynamics studies have revealed details of the transport pathway and some motions of EmrD at an atomic level (Baker et al. 2012).  Probably exports arabinose but not xylose (Koita and Rao 2012).

Accession Number:P31442
Protein Name:EmrD aka B3673
Length:394
Molecular Weight:42216.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate CCCP, H+, SDS, Benzalkonium

Cross database links:

Genevestigator: P31442
EchoBASE: EB1644
EcoGene: EG11693
eggNOG: COG0477
HEGENOM: HBG298506
RefSeq: AP_004119.1    NP_418129.2   
Entrez Gene ID: 948180   
Pfam: PF07690   
BioCyc: EcoCyc:EMRD-MONOMER    ECOL168927:B3673-MONOMER   
KEGG: ecj:JW5634    eco:b3673   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015238 F:drug transmembrane transporter activity
GO:0015893 P:drug transport
GO:0055085 P:transmembrane transport

References (5)

[1] “An E. coli gene emrD is involved in adaptation to low energy shock.”  Naroditskaya V.et.al.   8240355
[2] “DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.”  Burland V.D.et.al.   7686882
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
2GFP     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKRQRNVNLL LMLVLLVAVG QMAQTIYIPA IADMARDLNV REGAVQSVMG AYLLTYGVSQ 
61:	LFYGPISDRV GRRPVILVGM SIFMLATLVA VTTSSLTVLI AASAMQGMGT GVGGVMARTL 
121:	PRDLYERTQL RHANSLLNMG ILVSPLLAPL IGGLLDTMWN WRACYLFLLV LCAGVTFSMA 
181:	RWMPETRPVD APRTRLLTSY KTLFGNSGFN CYLLMLIGGL AGIAAFEACS GVLMGAVLGL 
241:	SSMTVSILFI LPIPAAFFGA WFAGRPNKRF STLMWQSVIC CLLAGLLMWI PDWFGVMNVW 
301:	TLLVPAALFF FGAGMLFPLA TSGAMEPFPF LAGTAGALVG GLQNIGSGVL ASLSAMLPQT 
361:	GQGSLGLLMT LMGLLIVLCW LPLATRMSHQ GQPV