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2.A.1.21.1
The macrolide (erythromycin; oleando-mycin; azithromycin; telithromycin) efflux, MefA, of 405 aas and 12 TMSs (Cantón et al. 2005; Bley et al. 2011).

Accession Number:P95827
Protein Name:MefA aka MEF
Length:405
Molecular Weight:44279.00
Species:Streptococcus pyogenes, and OS Streptococcus pneumoniae [1314]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate erythromycin, Oleandomycin, Azithromycin

Cross database links:

Pfam: PF05977   

References (3)

[1] “Molecular cloning and functional analysis of a novel macrolide-resistance determinant, mefA, from Streptococcus pyogenes.”  Clancy J.et.al.   8971709
[2] “The novel conjugative transposon tn1207.3 carries the macrolide efflux gene mef(A) in Streptococcus pyogenes.”  Santagati M.et.al.   12959402
[3] “Structure and distribution of an unusual chimeric genetic element encoding macrolide resistance in phylogenetically diverse clones of group A Streptococcus.”  Banks D.J.et.al.   14673771

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEKYNNWKLK FYTIWAGQAV SLITSAILQM AIIFYLTEKT GSAMVLSMAS LLGFLPYAVF 
61:	GPAIGVLVDR HDRKKIMIGA DLIIAAAGSV LTIVAFYMEL PVWMVMIVLF IRSIGTAFHT 
121:	PALNAVTPLL VPEEQLTKCA GYSQSLQSIS YIVSPAVAAL LYSVWELNAI IAIDVLGAVI 
181:	ASITVAIVRI PKLGDRVQSL DPNFIREMQE GMAVLRQNKG LFALLLVGTL YMFVYMPINA 
241:	LFPLISMDYF NGTPVHISIT EISFASGMLI GGLLLGLFGN YQKRILLITA SIFMMGISLT 
301:	ISGLLPQSGF FIFVVCCAIM GLSVPFYSGV QTALFQEKIK PEYLGRVFSL TGSIMSLAMP 
361:	IGLILSALFA DRIGVNHWFL LSGTLIICIA IVCPMINEIR KLDLK