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2.A.1.3.11
Puromycin:H+ antiporter

Accession Number:P42670
Protein Name:Pur8
Length:503
Molecular Weight:51852.00
Species:Streptomyces lipmanii (Streptomyces alboniger) [132473]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate puromycin, H+

Cross database links:

Pfam: PF07690   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015520 F:tetracycline:hydrogen antiporter activity
GO:0017000 P:antibiotic biosynthetic process
GO:0046677 P:response to antibiotic
GO:0015904 P:tetracycline transport
GO:0055085 P:transmembrane transport

References (1)

[1] “The pur8 gene from the pur cluster of Streptomyces alboniger encodes a highly hydrophobic polypeptide which confers resistance to puromycin.”  Tercero J.A.et.al.   7916693

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MARKPDISAV PVESAACQGP DPRRWWGLVV ILAAQLLVVL DGTVVNIALP SVQRDLGMSD 
61:	TSRQWVITAY TLAFGGLLLL GGRVADAFGR RRIFAVGILG FGLASLLGGA APDPGTLFLA 
121:	RALQGVFAAA LAPAALALIN TLFTEPGERG KAFGVYGAVS GGGAAVGLLA GGLLTEYLDW 
181:	RWCLYVNAPV ALLALLGCRL LPRDRRTGRA VRLDLPGTLL GCGGLVAIVY AFAEAESGWG 
241:	DPLVVRLLVL GVLMLVAFAL VERRVQDPLL PPGVVAHRVR GGSFLVVGLP QIGLFGLFLF 
301:	LTYYLQGILD YSPVLTGVAF LPLGLGIAVG SSLIAARLLP RTRPRTLIVG ALLAAAAGMA 
361:	LLTRLEPDTP QVYLTHLLPA QILIGLGIGC MMMPAMHTAT ARVAPHEAGA AAAVVNSAQQ 
421:	VGGALGVALL NTVSTGATAA YLADHGTSPA ATVDGTVHGY TVAIAFAVGV LLLTAVLAWV 
481:	LIDSRTEAAD ETGSASVTPA RPR