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2.A.1.3.16
The Me2+·tetracycline:2H+ antiporter
(Me2+ = Co2+, Mg2+, Mn2+) (also probably
a Na+ or K+:2H+ antiporter) (Wang et al. 2000).

Accession Number:P23054
Protein Name:TCRB aka TetA aka TETB aka TET
Length:458
Molecular Weight:49756.00
Species:Bacillus subtilis [1423]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate H+

Cross database links:

HEGENOM: HBG349047
RefSeq: NP_391957.1   
Entrez Gene ID: 937890   
Pfam: PF07690   
KEGG: bsu:BSU40770   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015520 F:tetracycline:hydrogen antiporter activity
GO:0015992 P:proton transport
GO:0046677 P:response to antibiotic
GO:0015904 P:tetracycline transport
GO:0055085 P:transmembrane transport

References (3)

[1] “Nucleotide sequence homology of the tetracycline-resistance determinant naturally maintained in Bacillus subtilis Marburg 168 chromosome and the tetracycline-resistance gene of B. subtilis plasmid pNS1981.”  Sakaguchi R.et.al.   2844262
[2] “Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin.”  Ogasawara N.et.al.   7584024
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNTSYSQSTL RHNQVLIWLC VLSFFSVLNE MVLNVSLPDI ANEFNKLPAS ANWVNTAFML 
61:	TFSIGTALYG KLSDQLGIKN LLLFGIMVNG LGSIIGFVGH SFFPILILAR FIQGIGAAAF 
121:	PALVMVVVAR YIPKENRGKA FGLIGSLVAM GEGVGPAIGG MVAHYIHWSY LLLIPTATII 
181:	TVPFLIKLLK KEERIRGHID MAGIILMSAG IVFFMLFTTS YRFSFLIISI LAFFIFVQHI 
241:	RKAQDPFVDP ELGKNVFFVI GTLCGGLIFG TVAGFVSMVP YMMKDVHHLS TAAIGSGIIF 
301:	PGTMSVIIFG YIGGLLVDRK GSLYVLTIGS ALLSSGFLIA AFFIDAAPWI MTIIVIFVFG 
361:	GLSFTKTVIS TVVSSSLKEK EAGAGMSLLN FTSFLSEGTG IAIVGGLLSI GFLDHRLLPI 
421:	DVDHSTYLYS NMLILFAGII VICWLVILNV YKRSRRHG