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2.A.1.3.2
Exporter of CCCP, nalidixic acid, rhodamine 6G, methylviologen, deoxycholate, growth inhibitory steroid hormones (estradiol and progesterone) (Elkins and Mullis, 2006) SDS, organomercurials, etc. (Nishino and Yamaguchi 2001).

Accession Number:P0AEJ0
Protein Name:Multidrug resistance protein B EmrB aka B2686
Length:512
Molecular Weight:55624.00
Species:Escherichia coli [83333]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate CCCP, Nalidixic acid, Rhodamine 6G, Methylviologen, Deoxycholate, estradiol, progesterone

Cross database links:

Genevestigator: P0AEJ0
EchoBASE: EB1409
EcoGene: EG11439
eggNOG: COG0477
HEGENOM: HBG759108
RefSeq: AP_003258.1    NP_417171.1   
Entrez Gene ID: 947167   
Pfam: PF07690   
BioCyc: EcoCyc:EMRB-MONOMER    ECOL168927:B2686-MONOMER   
KEGG: ecj:JW2661    eco:b2686   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0046677 P:response to antibiotic
GO:0055085 P:transmembrane transport

References (5)

[1] “Emr, an Escherichia coli locus for multidrug resistance.”  Lomovskaya O.et.al.   1409590
[2] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQQQKPLEGA QLVIMTIALS LATFMQVLDS TIANVAIPTI AGNLGSSLSQ GTWVITSFGV 
61:	ANAISIPLTG WLAKRVGEVK LFLWSTIAFA IASWACGVSS SLNMLIFFRV IQGIVAGPLI 
121:	PLSQSLLLNN YPPAKRSIAL ALWSMTVIVA PICGPILGGY ISDNYHWGWI FFINVPIGVA 
181:	VVLMTLQTLR GRETRTERRR IDAVGLALLV IGIGSLQIML DRGKELDWFS SQEIIILTVV 
241:	AVVAICFLIV WELTDDNPIV DLSLFKSRNF TIGCLCISLA YMLYFGAIVL LPQLLQEVYG 
301:	YTATWAGLAS APVGIIPVIL SPIIGRFAHK LDMRRLVTFS FIMYAVCFYW RAYTFEPGMD 
361:	FGASAWPQFI QGFAVACFFM PLTTITLSGL PPERLAAASS LSNFTRTLAG SIGTSITTTM 
421:	WTNRESMHHA QLTESVNPFN PNAQAMYSQL EGLGMTQQQA SGWIAQQITN QGLIISANEI 
481:	FWMSAGIFLV LLGLVWFAKP PFGAGGGGGG AH