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2.A.1.3.54
Protein SGE1 (10-N-nonyl acridine orange resistance protein) (Crystal violet resistance protein)

Accession Number:P33335
Protein Name:Protein SGE1
Length:543
Molecular Weight:59426.00
Species:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Crystal violet

Cross database links:

Genevestigator: P33335
eggNOG: COG0477
HEGENOM: HOG000247960
DIP: DIP-7575N
Entrez Gene ID: 856327   
Pfam: PF07690   
KEGG: sce:YPR198W   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0015238 F:drug transmembrane transporter activity

References (6)

[1] “Isolation and characterization of SGE1: a yeast gene that partially suppresses the gal11 mutation in multiple copies.”  Amakasu H.et.al.   8349103
[2] “The Saccharomyces cerevisiae SGE1 gene product: a novel drug-resistance protein within the major facilitator superfamily.”  Ehrenhofer-Murray A.E.et.al.   8058039
[3] “The Saccharomyces cerevisiae MFS superfamily SGE1 gene confers resistance to cationic dyes.”  Jacquot C.et.al.   9271105
[4] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.”  Bussey H.et.al.   9169875
[5] “The Sge1 protein of Saccharomyces cerevisiae is a membrane-associated multidrug transporter.”  Ehrenhofer-Murray A.E.et.al.   9483795
[6] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKSTLSLTLC VISLLLTLFL AALDIVIVVT LYDTIGIKFH DFGNIGWLVT GYALSNAVFM 
61:	LLWGRLAEIL GTKECLMISV IVFEIGSLIS ALSNSMATLI SGRVVAGFGG SGIESLAFVV 
121:	GTSIVRENHR GIMITALAIS YVIAEGVGPF IGGAFNEHLS WRWCFYINLP IGAFAFIILA 
181:	FCNTSGEPHQ KMWLPSKIKK IMNYDYGELL KASFWKNTFE VLVFKLDMVG IILSSAGFTL 
241:	LMLGLSFGGN NFPWNSGIII CFFTVGPILL LLFCAYDFHF LSLSGLHYDN KRIKPLLTWN 
301:	IASNCGIFTS SITGFLSCFA YELQSAYLVQ LYQLVFKKKP TLASIHLWEL SIPAMIATMA 
361:	IAYLNSKYGI IKPAIVFGVL CGIVGSGLFT LINGELSQSI GYSILPGIAF GSIFQATLLS 
421:	SQVQITSDDP DFQNKFIEVT AFNSFAKSLG FAFGGNMGAM IFTASLKNQM RSSQLNIPQF 
481:	TSVETLLAYS TEHYDGPQSS LSKFINTAIH DVFYCALGCY ALSFFFGIFT SSKKTTISAK 
541:	KQQ