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2.A.1.3.6
Me2+·tetracycline:2H+ or 2K+ antiporter
(the optimal Me2+ = Co2+) (Also transports Na+ or K+out in exchange for 2H+.)

Accession Number:P02983
Protein Name:Tetracycline resistance protein TCR2 aka TetK aka TET aka P101
Length:459
Molecular Weight:50695.00
Species:Staphylococcus aureus [1280]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Me2+-tetracycline, H+, K+, Na+

Cross database links:

RefSeq: NP_053795.1    YP_001649337.1   
Entrez Gene ID: 2598303    5850692   
Pfam: PF07690   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015520 F:tetracycline:hydrogen antiporter activity
GO:0015992 P:proton transport
GO:0046677 P:response to antibiotic
GO:0015904 P:tetracycline transport
GO:0055085 P:transmembrane transport

References (3)

[1] “Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus.”  Khan S.A.et.al.   6657777
[2] “Characterization of the tetracycline resistance gene of plasmid pT181 of Staphylococcus aureus.”  Mojumdar M.et.al.   3142848
[3] “The tet(K) gene of plasmid pT181 of Staphylococcus aureus encodes an efflux protein that contains 14 transmembrane helices.”  Guay G.G.et.al.   8234490

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MFSLYKKFKG LFYSVLFWLC ILSFFSVLNE MVLNVSLPDI ANHFNTTPGI TNWVNTAYML 
61:	TFSIGTAVYG KLSDYINIKK LLIIGISLSC LGSLIAFIGH NHFFILIFGR LVQGVGSAAF 
121:	PSLIMVVVAR NITRKKQGKA FGFIGSIVAL GEGLGPSIGG IIAHYIHWSY LLILPMITIV 
181:	TIPFLIKVMV PGKSTKNTLD IVGIVLMSIS IICFMLFTTN YNWTFLILFT IFFVIFIKHI 
241:	SRVSNPFINP KLGKNIPFML GLFSGGLIFS IVAGFISMVP YMMKTIYHVN VATIGNSVIF 
301:	PGTMSVIVFG YFGGFLVDRK GSLFVFILGS LSISISFLTI AFFVEFSMWL TTFMFIFVMG 
361:	GLSFTKTVIS KIVSSSLSEE EVASGMSLLN FTSFLSEGTG IAIVGGLLSL QLINRKLVLE 
421:	FINYSSGVYS NILVAMAILI ILCCLLTIIV FKRSEKQFE