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2.A.1.4.3
Glycerol-P:Pi antiporter (may function by a 'rocker switch' mechanism; Law et al., 2007). The 3-d structure is known (3.3Å resolution) (Huang et al., 2003; Lemieux et al., 2005; Lemieux, 2007).

Accession Number:P08194
Protein Name:GlpT aka B2240
Length:452
Molecular Weight:50310.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Glycerol-P

Cross database links:

Genevestigator: P08194
EchoBASE: EB0396
EcoGene: EG10401
eggNOG: COG2271
HEGENOM: HBG559184
RefSeq: AP_002837.1    NP_416743.1   
Entrez Gene ID: 946704   
Pfam: PF07690   
BioCyc: EcoCyc:GLPT-MONOMER    ECOL168927:B2240-MONOMER   
KEGG: ecj:JW2234    eco:b2240   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0015527 F:glycerol-phosphate:inorganic phosphate anti...
GO:0006071 P:glycerol metabolic process
GO:0015794 P:glycerol-3-phosphate transport
GO:0055085 P:transmembrane transport

References (7)

[1] “Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phosphate transport protein with components of the hexose-6-phosphate transport system.”  Eiglmeier K.et.al.   3329281
[2] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “The transmembrane topology of the sn-glycerol-3-phosphate permease of Escherichia coli analysed by phoA and lacZ protein fusions.”  Goett P.et.al.   3141744
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[7] “Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli.”  Huang Y.et.al.   12893936
Structure:
1PW4     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLSIFKPAPH KARLPAAEID PTYRRLRWQI FLGIFFGYAA YYLVRKNFAL AMPYLVEQGF 
61:	SRGDLGFALS GISIAYGFSK FIMGSVSDRS NPRVFLPAGL ILAAAVMLFM GFVPWATSSI 
121:	AVMFVLLFLC GWFQGMGWPP CGRTMVHWWS QKERGGIVSV WNCAHNVGGG IPPLLFLLGM 
181:	AWFNDWHAAL YMPAFCAILV ALFAFAMMRD TPQSCGLPPI EEYKNDYPDD YNEKAEQELT 
241:	AKQIFMQYVL PNKLLWYIAI ANVFVYLLRY GILDWSPTYL KEVKHFALDK SSWAYFLYEY 
301:	AGIPGTLLCG WMSDKVFRGN RGATGVFFMT LVTIATIVYW MNPAGNPTVD MICMIVIGFL 
361:	IYGPVMLIGL HALELAPKKA AGTAAGFTGL FGYLGGSVAA SAIVGYTVDF FGWDGGFMVM 
421:	IGGSILAVIL LIVVMIGEKR RHEQLLQERN GG