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2.A.1.42.1
The lysophospholipid transporter, LplT (Harvat et al., 2005).  Substrates include lyso-PE, lyso-cardiolipin, diacylcardiolipin, fully-deacylated cardiolipin and lyso-phosphatidylglycerol, but not lysophosphatidylcholine, lysophosphatidic acid or phosphatidic acid (Lin et al. 2016). Reacylation by acyltransferase/acyl-acyl carrier protein synthetase then occurs on the inner leaflet of the membrane.Thus, a fatty acid chain is not required for LplT transport. A "sideways sliding" mechanism was proposed to explain how a conserved membrane-embedded α-helical interface excludes diacylphospholipids from the LplT binding site to facilitate efficient flipping of lysophospho-lipids across the cell membrane (Lin et al. 2016). Thus, a fatty acid chain is not required for LplT transport. Fruther, LplT cannot transport lysophosphatidic acid, and its substrate binding was not inhibited by either orthophosphate or glycerol 3-phosphate, indicating that either a glycerol or ethanolamine headgroup is the chemical determinant for substrate recognition. Diacyl forms of PE, phosphatidylglycerol, or the tetra-acylated form of cardiolipin could not serve as competitive inhibitors .A "sideways sliding" mechanism was proposed to explain how a conserved membrane-embedded α-helical interface can exclude diacylphospholipids from the LplT binding site.

Accession Number:P39196
Protein Name:FAP aka YgeD
Length:397
Molecular Weight:41656.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Lyso-PE, Lyso-Cardiolipin, Diacylcardiolipin, Fully-deacetylated Cardiolipin, lyso-phosphatidylglycerol

Cross database links:

Genevestigator: P39196
EchoBASE: EB2349
EcoGene: EG12455
eggNOG: COG0477
HEGENOM: HBG364376
RefSeq: AP_003398.1    NP_417312.1   
Entrez Gene ID: 947317   
Pfam: PF07690   
BioCyc: EcoCyc:EG12455-MONOMER    ECOL168927:B2835-MONOMER   
KEGG: ecj:JW2803    eco:b2835   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005319 F:lipid transporter activity
GO:0006869 P:lipid transport
GO:0055085 P:transmembrane transport

References (5)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Sequence and function of the aas gene in Escherichia coli.”  Jackowski S.et.al.   8300626
[4] “Lysophospholipid flipping across the Escherichia coli inner membrane catalyzed by a transporter (LplT) belonging to the major facilitator superfamily.”  Harvat E.M.et.al.   15661733
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

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  • CDD Search (Conserved Domain Database)
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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSESVHTNTS LWSKGMKAVI VAQFLSAFGD NALLFATLAL LKAQFYPEWS QPILQMVFVG 
61:	AYILFAPFVG QVADSFAKGR VMMFANGLKL LGAASICFGI NPFLGYTLVG VGAAAYSPAK 
121:	YGILGELTTG SKLVKANGLM EASTIAAILL GSVAGGVLAD WHVLVALAAC ALAYGGAVVA 
181:	NIYIPKLAAA RPGQSWNLIN MTRSFLNACT SLWRNGETRF SLVGTSLFWG AGVTLRFLLV 
241:	LWVPVALGIT DNATPTYLNA MVAIGIVVGA GAAAKLVTLE TVSRCMPAGI LIGVVVLIFS 
301:	LQHELLPAYA LLMLIGVMGG FFVVPLNALL QERGKKSVGA GNAIAVQNLG ENSAMLLMLG 
361:	IYSLAVMIGI PVVPIGIGFG ALFALAITAL WIWQRRH