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2.A.1.48.5
Originally considered to be vacuolar basic amino acid transporter 4, but it my not act on amino acids, but exports drugs such as azoles.  May also play a role in vacuolar morphology (Kawano-Kawada et al. 2015).

Accession Number:Q04602
Protein Name:Vacuolar basic amino acid transporter 4
Length:768
Molecular Weight:85683.00
Species:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Azloes

Cross database links:

Genevestigator: Q04602 Q04602
eggNOG: NOG268386 NOG268386
DIP: DIP-8662N DIP-8662N
Entrez Gene ID: 851697    851697   
Pfam: PF07690    PF07690   
KEGG: sce:YDR119W    sce:YDR119W   

Gene Ontology

GO:0000329 C:fungal-type vacuole membrane
GO:0016021 C:integral to membrane
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport
GO:0000329 C:fungal-type vacuole membrane
GO:0016021 C:integral to membrane
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport

References (18)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[2] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[3] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[4] “A family of basic amino acid transporters of the vacuolar membrane from Saccharomyces cerevisiae.”  Shimazu M.et.al.   15572352
[5] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[6] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[7] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[8] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956
[9] “The yeast vacuolar membrane proteome.”  Wiederhold E.et.al.   19001347
[10] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[11] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[12] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[13] “A family of basic amino acid transporters of the vacuolar membrane from Saccharomyces cerevisiae.”  Shimazu M.et.al.   15572352
[14] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[15] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[16] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[17] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956
[18] “The yeast vacuolar membrane proteome.”  Wiederhold E.et.al.   19001347

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGKKDRQRKK LREFAKLKNR QRNLRKSVQT LKNEVQREAK VPRTSNQIAL GNDKIEEINE 
61:	NSPLLSAPSK QEEVSIPKAV DIDTIDAQPL HEGPKIDDSP QDEVNSIKGK PADKANEDDL 
121:	KPPSQHEACG NSALQSSITD FSDRSVSPLQ SITSCNTPMS EHELPVSSSN SFERADDMPV 
181:	VQADNQTSSS KSLHIVAPSP EVPVSGDEIT SYGYGSIPQS IGDVENGLNP PYVENTSSDE 
241:	LVHDLTRRRI FSSCMCTYLF FIAMDSSIIL VIASKIASEF HELWRLSLVI SAYLLSNAIG 
301:	QLVFLKLSLI SSVKLLLCIA QFSFILGGYL SWSSAHFWTF IFARCVTGFG GGSLIALKST 
361:	IMNRFSQKND SRYSLSASMI TFAMGVVIGP FMMNLFDSSH GSGWRNAFLI PVPFCLVNAS 
421:	IMLADMYSVK STLYGRPTPT LWKRFKNTLL SPDLYEILTL TLFLLCFVQV TSLDLTGLKN 
481:	NTMIQALLFS VIIVCGILFF LIETSDTYMN SVISMSLQGD KRLIWTMIGI SFCFAALMCI 
541:	IPFGTTYFII VLNLSTLQLA ERLSPFFFSI VLGYFSVSYF WKSKGQNFLL KFVLSGATLL 
601:	LYVALMGVSL NLPVWKQYIC LSLPFLGSSM ILTLLSNLYH EYHEQRKSPI SGSIVYCFGA 
661:	VGGTVGISLG GYVFHKTLIK LMHEKVMPFS KQGYLKKDLL KIIKHATESS DWVHESAPKF 
721:	VFQTLIECYL QACRNVFKLS TLFFTITVVA IFIFNRIHCR SQNCLSLS