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2.A.1.6.4
(Proline/glycine-betaine):(H+/Na+) symporter, ProP (also transports taurine, ectoine, pipecolate, proline-betaine, N,N-dimethylglycine, carnitine, and 1-carboxymethyl-pyridinium) (subject to osmotic activation). Transmembrane helix I and periplasmic loop 1 are involved in osmosensing and osmoprotectant transport (Keates et al., 2010).

Accession Number:P0C0L7
Protein Name:Proline/betaine transporter ProP aka B4111
Length:500
Molecular Weight:54846.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Glycine betaine, H+, Na+, Proline

Cross database links:

Genevestigator: P0C0L7
EchoBASE: EB1569
EcoGene: EG11612
eggNOG: COG0477
HEGENOM: HBG757988
RefSeq: AP_004612.1    NP_418535.1   
Entrez Gene ID: 948626   
Pfam: PF08946    PF00083   
BioCyc: EcoCyc:PROP-MONOMER    ECOL168927:B4111-MONOMER   
KEGG: ecj:JW4072    eco:b4111   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015295 F:solute:hydrogen symporter activity
GO:0015292 F:uniporter activity
GO:0042538 P:hyperosmotic salinity response
GO:0015824 P:proline transport
GO:0055085 P:transmembrane transport

References (6)

[1] “Isolation and sequencing of Escherichia coli gene proP reveals unusual structural features of the osmoregulatory proline/betaine transporter, ProP.”  Culham D.E.et.al.   8421314
[2] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[6] “Solution structure of the C-terminal antiparallel coiled-coil domain from Escherichia coli osmosensor ProP.”  Zoetewey D.L.et.al.   14643666
Structure:
1R48   1Y8S     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD 
61:	PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYD 
121:	TIGIWAPILL LICKMAQGFS VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA 
181:	GVVVLISTIV GEANFLDWGW RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE 
241:	GLQDGPKVSF KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI 
301:	IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV IGLIFAGLLM 
361:	LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA GLTPTLAAWL VESSQNLMMP 
421:	AYYLMVVAVV GLITGVTMKE TANRPLKGAT PAASDIQEAK EILVEHYDNI EQKIDDIDHE 
481:	IADLQAKRTR LVQQHPRIDE