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2.A.1.6.6
Shikimate:H+ symporter

Accession Number:P76350
Protein Name:ShiA aka B1981
Length:438
Molecular Weight:47817.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Shikimate, H+

Cross database links:

Genevestigator: P76350
EchoBASE: EB4148
EcoGene: EG20205
eggNOG: COG0477
HEGENOM: HBG757988
RefSeq: AP_002585.1    NP_416488.1   
Entrez Gene ID: 946495   
Pfam: PF00083   
BioCyc: EcoCyc:SHIA-MONOMER    ECOL168927:B1981-MONOMER   
KEGG: ecj:JW1962    eco:b1981   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015293 F:symporter activity
GO:0055085 P:transmembrane transport

References (5)

[1] “Cloning and analysis of the shiA gene, which encodes the shikimate transport system of Escherichia coli K-12.”  Whipp M.J.et.al.   9524262
[2] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDSTLISTRP DEGTLSLSRA RRAALGSFAG AVVDWYDFLL YGITAALVFN REFFPQVSPA 
61:	MGTLAAFATF GVGFLFRPLG GVIFGHFGDR LGRKRMLMLT VWMMGIATAL IGILPSFSTI 
121:	GWWAPILLVT LRAIQGFAVG GEWGGAALLS VESAPKNKKA FYSSGVQVGY GVGLLLSTGL 
181:	VSLISMMTTD EQFLSWGWRI PFLFSIVLVL GALWVRNGME ESAEFEQQQH YQAAAKKRIP 
241:	VIEALLRHPG AFLKIIALRL CELLTMYIVT AFALNYSTQN MGLPRELFLN IGLLVGGLSC 
301:	LTIPCFAWLA DRFGRRRVYI TGTLIGTLSA FPFFMALEAQ SIFWIVFFSI MLANIAHDMV 
361:	VCVQQPMFTE MFGASYRYSG AGVGYQVASV VGGGFTPFIA AALITYFAGN WHSVAIYLLA 
421:	GCLISAMTAL LMKDSQRA