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2.A.1.6.9
YdfJ. Can function as an inward rectifying K+ channel when expressed in animal cells as measured by whole cell patch clamping. Blocked by barium and protopine (Tang et al., 2011).

Accession Number:P77228
Protein Name:Putative inner membrane metabolite transport protein ydfJ
Length:427
Molecular Weight:46267.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

Genevestigator: P77228
EchoBASE: EB3583
EcoGene: EG13822
HEGENOM: HBG757988
DIP: DIP-11699N
Pfam: PF00083   
KEGG: ecj:JW1536   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015293 F:symporter activity

References (4)

[1] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDFQLYSLGA ALVFHEIFFP ESSTAMALIL AMGTYGAGYV ARIVGAFIFG KMGDRIGRKK 
61:	VLFITITMMG ICTTLIGVLP TYAQIGVFAP ILLVTLRIIQ GLGAGAEISG AGTMLAEYAP 
121:	KGKRGIISSF VAMGTNCGTL SATAIWAFMF FILSKEELLA WGWRIPFLAS VVVMVFAIWL 
181:	RMNLKESPVF EKVNDSNQPT AKPAPAGSMF QSKSFWLATG LRFGQAGNSG LIQTFLAGYL 
241:	VQTLLFNKAI PTDALMISSI LGFMTIPFLG WLSDKIGRRI PYIIMNTSAI VLAWPMLSII 
301:	VDKSYAPSTI MVALIVIHNC AVLGLFALEN ITMAEMFGCK NRFTRMAISK EIGGLIASGF 
361:	GPILAGIFCT MTESWYPIAI MIMAYSVIGL ISALKMPEVK DRDLSALEDA AEDQPRVVRA 
421:	AQPSRSL