TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.1.75.1
Probable transporter MCH1.  Although the name, "monocarboxylate transporter homologue 1" implies that this system transports monocarboxylates such as lactate, pyruvate and acetate, no evidence for this possibility was obtained (Makuc et al. 2001). Instead, the mch1-5 mutant strain, lacking all 5 such paralogues in yeast showed strongly reduced biomass yields in aerobic glucose-limited chemostat cultures, pointing to the involvement of Mch transporters in mitochondrial metabolism. Indeed, intracellular localization studies indicated that at least some of the Mch proteins reside in intracellular membranes.Thus, the yeast monocarboxylate transporter-homologs perform other functions other than do their mammalian counterparts (Makuc et al. 2001). Possibly they function in intracellular, organellar transport of these acids.

Accession Number:Q07376
Protein Name:Probable transporter MCH1
Length:486
Molecular Weight:53566.00
Species:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Monocarboxylates

Cross database links:

Genevestigator: Q07376
eggNOG: NOG314560
HEGENOM: HOG000246467
DIP: DIP-5410N
Entrez Gene ID: 851506   
Pfam: PF07690   
KEGG: sce:YDL054C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0055085 P:transmembrane transport

References (5)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[2] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[3] “The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane.”  Makuc J.et.al.   11536335
[4] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[5] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MPLSKVEHYL SYHTRLLLPH VLSLQSSHRV AYIFSLLSAV STGFITLISL YSQPWQKHLN 
61:	YSSWQINTIA SMTNLGMYLT PPILGMIADS HGPITLSLLA IIGFIPSYSY LAYVFNHPEL 
121:	SLGGNGDSSF NLSIICFVFI GISTSALYFS ALLTCTKLYP HTKLLSISLP TTCYGISSVV 
181:	GSQLLRIKWF WSSNASSSSS NSDLNLGRVF QTFALVYVVI GLLAWIATSV VSLLHFNEEQ 
241:	DNQKRLDDQT DVEQSPLLER SNHVQEKFTQ TMLRIFSDPV TYILAVSILL SLGPLEMFIA 
301:	NMGSLTNLLV QLDAPTLSTK LLSTYALSST FTRLLTGIVA DFFAKKKISI KWILLTFLSL 
361:	GVCAQLFLLK MTSSASPWGL VPTGSLVGIV YGGLFTVYPT LVLLVWGERS FGTVYGSLLI 
421:	APAIGSMIFC MLYAKFYDSR CMSGGGDLRN PSCISAVYKY SSIAFVVSAV LSAVVFWKLK 
481:	SRKLRI