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2.A.1.8.10
NO3-/NO2- transporter (NO3- uptake permease; NO2- exporter) (probable NO3-/NO2- antiporter) (stress-induced; Clegg et al., 2006; Jia et al. 2009)

Accession Number:P37758
Protein Name:NarU aka B1469
Length:462
Molecular Weight:49890.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate nitrate, Nitrite

Cross database links:

Genevestigator: P37758
EchoBASE: EB2073
EcoGene: EG12153
eggNOG: COG2223
HEGENOM: HBG307423
RefSeq: AP_002092.1    NP_415986.1   
Entrez Gene ID: 945799   
Pfam: PF07690   
BioCyc: EcoCyc:NARU-MONOMER    ECOL168927:B1469-MONOMER   
KEGG: ecj:JW1464    eco:b1469   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005452 F:inorganic anion exchanger activity
GO:0015112 F:nitrate transmembrane transporter activity
GO:0015514 F:nitrite efflux transmembrane transporter ac...
GO:0042128 P:nitrate assimilation
GO:0015706 P:nitrate transport
GO:0015707 P:nitrite transport
GO:0055085 P:transmembrane transport

References (6)

[1] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon.”  Blasco F.et.al.   2233673
[5] “Nitrate reductases in Escherichia coli.”  Bonnefoy V.et.al.   7747940
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
4IU8   4IU9     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MALQNEKNSR YLLRDWKPEN PAFWENKGKH IARRNLWISV SCLLLAFCVW MLFSAVTVNL 
61:	NKIGFNFTTD QLFLLTALPS VSGALLRVPY SFMVPIFGGR RWTVFSTAIL IIPCVWLGIA 
121:	VQNPNTPFGI FIVIALLCGF AGANFASSMG NISFFFPKAK QGSALGINGG LGNLGVSVMQ 
181:	LVAPLVIFVP VFAFLGVNGV PQADGSVMSL ANAAWIWVPL LAIATIAAWS GMNDIASSRA 
241:	SIADQLPVLQ RLHLWLLSLL YLATFGSFIG FSAGFAMLAK TQFPDVNILR LAFFGPFIGA 
301:	IARSVGGAIS DKFGGVRVTL INFIFMAIFS ALLFLTLPGT GSGNFIAFYA VFMGLFLTAG 
361:	LGSGSTFQMI AVIFRQITIY RVKMKGGSDE QAHKEAVTET AAALGFISAI GAVGGFFIPQ 
421:	AFGMSLNMTG SPVGAMKVFL IFYIVCVLLT WLVYGRRKFS QK