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2.A.1.8.12
The root cortical and epidermal cell, high affinity, plasma membrane, NO3- uptake transporter, NRT2.1 (Wirth et al., 2007). Also functions in nitrate sensing and signaling (Miller et al., 2007; Girin et al., 2010). Activity only occurs when NRT2.1 is complexed with NAR2.1 (WR3; 8.A.20.1.1) in a 2:2 tetrameric complex (Yong et al., 2010). NAR2.1 has an N-terminal and a C-terminal TMS and has been annotated as a calcineurin-like phosphoesterase family member (Yong et al., 2010).  Ntr transporters may also play a role in gaseous NO2 uptake by leaves (Hu et al. 2014).  The Medicago truncatula orthologue has been characterized (Pellizzaro et al. 2014).

Accession Number:O82811
Protein Name:High-affinity nitrate transporter ACH1 aka NRT2.1
Length:530
Molecular Weight:57710.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Endoplasmic reticulum membrane1 / Multi-pass membrane protein2
Substrate nitrate

Cross database links:

Genevestigator: O82811
eggNOG: euNOG04277
HEGENOM: HBG617617
RefSeq: NP_172288.1   
Entrez Gene ID: 837327   
Pfam: PF07690   
KEGG: ath:AT1G08090   

Gene Ontology

GO:0015112 F:nitrate transmembrane transporter activity
GO:0048527 P:lateral root development
GO:0015706 P:nitrate transport
GO:0010167 P:response to nitrate
GO:0055085 P:transmembrane transport

References (3)

[1] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[2] “Nitrate transport: a key step in nitrate assimilation.”  Daniel-Vedele F.et.al.   10066586
[3] “Expression analysis of a high-affinity nitrate transporter isolated from Arabidopsis thaliana by differential display.”  Filleur S.et.al.   9951738

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGDSTGEPGS SMHGVTGREQ SFAFSVQSPI VHTDKTAKFD LPVDTEHKAT VFKLFSFAKP 
61:	HMRTFHLSWI SFSTCFVSTF AAAPLVPIIR ENLNLTKQDI GNAGVASVSG SIFSRLVMGA 
121:	VCDLLGPRYG CAFLVMLSAP TVFSMSFVSD AAGFITVRFM IGFCLATFVS CQYWMSTMFN 
181:	SQIIGLVNGT AAGWGNMGGG ITQLLMPIVY EIIRRCGSTA FTAWRIAFFV PGWLHIIMGI 
241:	LVLNLGQDLP DGNRATLEKA GEVAKDKFGK ILWYAVTNYR TWIFVLLYGY SMGVELSTDN 
301:	VIAEYFFDRF HLKLHTAGLI AACFGMANFF ARPAGGYASD FAAKYFGMRG RLWTLWIIQT 
361:	AGGLFCVWLG RANTLVTAVV AMVLFSMGAQ AACGATFAIV PFVSRRALGI ISGLTGAGGN 
421:	FGSGLTQLLF FSTSHFTTEQ GLTWMGVMIV ACTLPVTLVH FPQWGSMFLP PSTDPVKGTE 
481:	AHYYGSEWNE QEKQKNMHQG SLRFAENAKS EGGRRVRSAA TPPENTPNNV