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2.A.1.8.2
Nitrate uptake porter

Accession Number:P42432
Protein Name:NasA
Length:401
Molecular Weight:42712.00
Species:Bacillus subtilis [1423]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate nitrate, Nitrite

Cross database links:

HEGENOM: HBG479449
RefSeq: NP_388215.2   
Entrez Gene ID: 938326   
Pfam: PF07690   
KEGG: bsu:BSU03330   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015103 F:inorganic anion transmembrane transporter a...
GO:0015698 P:inorganic anion transport
GO:0042128 P:nitrate assimilation
GO:0055085 P:transmembrane transport

References (6)

[1] “The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.”  Ogawa K.et.al.   7868621
[2] “The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes.”  Yamane K.et.al.   8969502
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[4] “From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.”  Barbe V.et.al.   19383706
[5] “Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor.”  Wray L.V. Jr.et.al.   10864496
[6] “Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.”  Yoshida K.et.al.   12823818

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKLSELKTSG HPLTLLCSFL YFDVSFMIWV MLGALGVYIS QDFGLSPFEK GLVVAVPILS 
61:	GSVFRIILGI LTDRIGPKKT AVIGMLVTMI PLLWGTFGGR SLTELYAIGI LLGVAGASFA 
121:	VALPMASRWY PPHLQGLAMG IAGAGNSGTL FATLFGPRLA EQFGWHIVMG IALIPLLIVF 
181:	ILFVSMAKDS PAQPSPQPLK SYLHVFGQKE TWFFCLLYSV TFGGFVGLSS FLSIFFVDQY 
241:	QLSKIHAGDF VTLCVAAGSF FRPVGGLISD RVGGTKVLSV LFVIVALCMA GVSSLPSLSM 
301:	VIVLLFVGMM GLGMGNGAVF QLVPQRFRKE IGMVTGIVGA AGGIGGFFLP NILGSLKQMT 
361:	GTYAIGFITF SCIALLAFAL VLAAGYYWRK SWSAESSPAD V